6. Instrument Features
2016-04
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
145
Note
AlphaScreen/AlphaLISA measurements are only possible as endpoint
measurements in white or light gray microplates and cannot be
performed in combination with the injector system and the heating
system.
Parameters
Characteristics
Wavelength Range:
380 - 700 nm
Standard Filters
AlphaScreen:
Excitation: 680 (30) nm
Emission: 570 (100) nm
Standard Filters
AlphaLISA:
Excitation 680 (30) nm
Emission: 615 (20) nm
Gain Setting
Values
Measurement Range
Manual
1 - 255
0 - 60,000 RFU
Calculated from
Well
automatic
0 - 60,000 RFU
Parameters
Characteristics
Excitation time:
100 - 2000 ms (in 10 ms steps)
Integration time:
100 - 2000 ms (in 10 ms steps)
6.10 “On the Fly” Measurements
“On the Fly” measurements are the fastest measurements possible using the
Infinite 200 PRO. These measurements are performed using only one flash
(number of flashes).
96-well plates (FI, TRF, Absorbance) Measurement time: < 20 s
384-well plates (FI, TRF, Absorbance) Measurement time: < 30 s
(Plate-in/out movement not included).
