5. Operating the Instrument
108
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
2016-04
5.10 Optimizing AlphaScreen/AlphaLISA
Measurements
CAUTION
SWITCH ON THE INSTRUMENT AT LEAST 15 MINUTES BEFORE
STARTING AN ALPHASCREEN/ALPHALISA MEASUREMENT TO ENSURE
STABLE CONDITIONS FOR THE MEASUREMENT.
Note
AlphaScreen/AlphaLISA measurements are only possible as endpoint
measurements in white or light gray microplates and cannot be
performed in combination with the injector system and the heating
system.
5.10.1 Instrument Parameters
Gain Settings
The Infinite 200 PRO AlphaScreen/AlphaLISA detection system uses an analog
to digital (ADC: Analog Digital Converter) conversion of PMT signal. The gain
setting controls the amplification of the PMT when converting light into electrical
current. The ADC needs a suitable input range of PMT current to provide a
proper signal to noise ratio (S/N) on the one hand, and linearity on the other
hand. Therefore, the gain should be tuned to make highest concentration
microplate wells give highest possible readings. Then, readings of microplate
wells that contain lower concentrations can be effectively separated from
background signals - as far as the background noise level allows for that.
Note
If any well of interest is assigned “OVER” (signal overflow), you may
manually reduce the gain, or select the ‘calculated from well’ gain
option (see the software Instructions for Use).
When the ‘calculated from well’ gain option is used, the instrument will require a
waiting time during the gain optimization to properly adjust the gain for the
subsequent sample measurement. To improve the total measurement speed, the
gain may be determined in a pre-measurement using a well containing the
highest signal/concentration and then set manually before the whole plate is read.
The gain should be adjusted so that the wells with the maximum signal yield app.
55000 RFU.
STOP
