5. Operating the Instrument
2016-04
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
97
5.6.3
G-Factor Settings
The given equation for calculation of fluorescence polarization assumes that the
sensitivity of the detection system is equivalent for parallel and perpendicular
polarized light. This is generally not the case and either the parallel or
perpendicular intensity must be corrected by so called ‘G-Factor’. The G-factor
compensates for differences in optical components between parallel and
perpendicular measurement.
The G-Factor is the correction factor that can be determined for the wavelength of
the fluorophore by measuring a sample with a known polarization value. A valid
calibration of the instrument resulting in a G-factor is an important requirement for
each fluorescence polarization measurement.
CAUTION
MAKE SURE THAT THE FILTER SLIDE CONTAINS POLARIZERS
TOGETHER WITH THE FILTERS DEFINED FOR FLUORESCENCE
POLARIZATION. MEASUREMENTS WITHOUT THE POLARIZERES WILL
RESULT IN A FALSE G-FACTOR AND FALSE MEASUREMENT DATA.
5.6.4
Measurement with an Uncalibrated G-Factor
If no calibrated G-factor is available, the default value of 1 will be displayed and
marked as ‘Uncalibrated G-Factor’. In order to enable the measurement, confirm
this value or select a new one by either clicking the up and down arrows or
entering a value in the
G-Factor
field.
For the G-Factor calibration, see 5.6.5.
STOP
