5. Operating the Instrument
130
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
2016-04
Example 2: Enliten
®
ATP Assay System
Bioluminescence Detection Kit for ATP (Promega Corp.)
For assay details please refer to www.promega.com.
Plate
Select an appropriate plate type. For luminescence measurements, white
microplates are recommended. For this example, a white 96 well plate was
selected.
Part of the plate
(Not shown); can be optionally selected if only part of the plate should be
processed
Well
Mandatory for measurements with ‘injection’
Injection
Injector A injects 100 µl with speed 100 µl/sec., refill mode: standard
Wait (Timer)
2 s wait time
Luminescence
Luminescence measurement
with 10 s integration time, attenuation ‘none’
Move Plate
Plate is moved out after finishing all wells
CAUTION
SWITCH ON THE INSTRUMENT AT LEAST 15 MINUTES BEFORE
STARTING A LUMINESCENCE MEASUREMENT. SOME COMPONENTS
NEED TO WARM UP TO GUARANTEE STABLE CONDITIONS FOR THE
MEASUREMENT.
STOP
