7. Quality Control
2016-04
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
169
7.3.5
Glow Luminescence
For the Infinite 200 PRO with the option ‘Luminescence the following tests may
be performed to prove the sensitivity specifications:
Sensitivity
Perform the following measurement to determine the sensitivity:
Measurement Parameters:
Parameter
Setting
Reading Mode
Luminescence
Integration Time
1000 ms
Settle Time
0
Plate Type
GRE96fw
Part of the Plate
A1 – D10
Plate Layout:
Pipette 200 µl of the ATP reagents into the appropriate wells according to the
plate layout:
1
2
3
4
5
6
7
8
9
10
11
12
A
Bx
ATP
Bx
B
B
B
B
B
B
B
B
Bx
ATP
Bx
B
B
B
B
B
B
B
C
Bx
ATP
Bx
B
B
B
B
B
B
B
D
Bx
ATP
Bx
B
B
B
B
B
B
B
E
F
G
H
ATP
2*10
-8
M ATP (final concentration in well)
B
Blank (ATP reagent: Tris-EDTA=1:5)
Bx
Blank (wells used for cross-talk calculation)
Material/Reagents:
BioThema ATP Kit (ATP-Kit SL 144-041, BioThema AB)
1 Greiner 96-well plate white
200 µl P tips
