6. Instrument Features
140
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
2016-04
6.4
Fluorescence Polarization (FP)
Infinite F200 PRO only
Note
The option ‘Fluorescence Polarization’ is only available for the Infinite
F200 PRO. This module cannot be installed on an Infinite M200 PRO.
Parameters
Instrument type
Characteristics
Wavelength
Range
standard
Excitation: 300 – 600 nm
spectrally-
enhanced
Excitation: 300 – 850 nm
standard
Emission: 330 – 600 nm
spectrally-
enhanced
Emission: 330 – 850 nm
Standard Filter
both
Configuration of Default filter
slide:
Exc 485 (20) nm
Position on Exc filter slide:
Exc1: 485 (20) - parallel
Exc2: 485 (20) - perpendicular
Em 535 (25) nm
Position on Em filter slide:
Em1: 535 (25) - parallel
Em2: 535 (25) - parallel
Gain Setting
Values
Measurement Range
Manual
1 - 255
0 - 60,000 RFU
Optimal
automatic
0 - 60,000 RFU
Calculated from Well
automatic
0 - 60,000 RFU
FP Parameters
Characteristics
Integration Time
20 - 2000 µs
Lag Time
0 - 2000 µs
FP Precision (F200 only)
< 5 mP @ 1nM Fluorescein