Chapter 7
Tips on Photographing
53
Photogr
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Basics of DS-L3
(5) Adjusting
the
lighting
When photographing a color image, the color reproducibility of the image depends on the lamp voltage.
When a halogen lamp is used as the light source, increasing the lamp voltage produces a bluish light, while decreasing the
lamp voltage produces a reddish light.
Except in cases where it is specifically necessary to adjust the tone, the voltage should be set to the proper level for
microphotography, which depends on the microscope being used. Since this level varies for each type of microscope, refer to
your microscope’s instruction manual.
Note: If the lamp voltage changes, adjust the white balance once again.
White balance when observing by a microscope
Consider the following when you adjust the white balance while viewing on the microscope.
•
When using diascopic illumination for microphotography, adjust the white balance while you are photographing a
transparent part of the preparation.
•
When using episcopic illumination for microphotography or using a lens, adjust the white balance using a white
subject.
•
For fluorescent photographing, we recommend that the white balance be adjusted under normal light conditions
before photographing. If the screen is excessively dark or bright, adjust the luminous energy of the light source or
the iris diaphragm, or use an ND filter to obtain the appropriate white balance.
(6) Adjusting the exposure time
An exposure time in the range of 60 ms to 4 ms is appropriate for a normal photographing. Adjust the light intensity for the
microscope using an ND filter to get an appropriate exposure time.
When automatic exposure is set for DS-L3, the camera gain and the exposure time are automatically changed to obtain an
appropriate exposure. For this reason, a weak light intensity causes a high camera gain making the image coarse, and a long
exposure time making the focusing difficult.
What is an ND filter?
An ND filter is a filter that affects only the amount of light passed, not the color balance of the light. For example, an
“ND2” filter cuts transmitted light in half, while an “ND16” filter reduces transmitted light to 1/16th of actual levels.
Summary of Contents for DS-L3
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