Chapter 7
Tips on Photographing
50
Photogr
ap
hin
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Basics of DS-L3
7
Tips on Photographing
This chapter provides useful tips for photographing.
7.1
Checking the Image Condition
7.1.1
Checking the Histogram of the Image
When you press the [HISTOGRAM] button on the task bar, the
[HISTOGRAM] window appears at the right bottom of the screen.
On the [INFO: HISTOGRAM] menu, the system calculates the
distribution of brightness of the image and shows it as a
histogram.
The histogram indicates distribution of brightness for each RGB
color which allows fine adjustment of the exposure level.
•
The horizontal axis indicates brightness (dark to light from left
to right).
•
The vertical axis indicates appearance frequency.
•
On the right to the histogram, average values of RGB colors
are shown.
Dark
Brightness
Bright
[INFO: HISTOGRAM] menu
Reading and compensating the exposure
When the distribution reaches the right end of the histogram, the bright part of the image is saturated (white-out).
When the distribution reaches the left end of the histogram, the dark part of the image is collapsed (black-out).
It is preferred that no white-out and black-out occurs in the entire image, but, in some cases, adjustment is
necessary.
If a bright part of the image is important, adjust the distribution to the left. If a dark part is important, adjust the
distribution to the right.
By adjusting the distribution in accordance with the important level, it is possible to prevent insufficient gradation
during image processing.
7.1.2
Checking the Microscope Condition
When DS-L3 and a Nikon microscope are connected by a USB
cable, the title section of the [INFO: HISTOGRAM] menu shows a
submenu, by which you can switch the screen to the [INFO: MIC
INFO] menu.
The [INFO: MIC INFO] menu shows the microscope information,
by which you can confirm the microscope condition on the screen.
For the content of the [MICROSCOPE INFORMATION] screen,
refer to a separate volume of this manual, “Microscope
Operation”.
[INFO: MIC INFO] menu
Tips on Photographing
7
Intensity
Average
for each
color of
RGB
Microscope
conditions
Chapter
Summary of Contents for DS-L3
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