Thermo Scientific DSQ II, Руководство по оборудованию

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1
Basic Operations
Installing a Column into the GC/MS System
Thermo Scientific DSQ II Hardware Manual 5
a. Unwind the column about a half turn for the inlet and two turns for the DSQ II.
b. Wipe about 100 mm (4 in.) of the column with a tissue soaked in methanol.
c. Insert the column through the injector nut and ferrule (open end up). Wipe the
column again with a tissue soaked in methanol.
d. Use a scoring wafer to score and break the column about 2.5 cm (1 in.) from the end.
Use a magnifying glass to check for an even, flat cut. Repeat if necessary.
e. Insert the column into the injector so that the end of the column is the proper
distance (splitless = 64 mm, split = 40 mm, PTV = 30 mm) from the back of the
injector nut.
f. Verify the position with the column measuring tool.
g. Use your fingers to tighten the injector nut and give it an additional quarter turn
with the wrench.
h. Use a scoring wafer to score and break the column outlet about 2.5 cm (1 in.) from
the end.
i. Power on the TRACE GC Ultra.
2. Set up the TRACE GC Ultra.
a. Set the oven and injector temperatures to 30 °C.
b. Set the injector flow to 1.0 mL/min.
c. Turn off vacuum compensation (under the Right or Left Carrier menu).
d. Dip the column outlet in a small vial of methanol. Bubbles indicate there is flow
through the column.
e. Allow the column to purge for at least 10 minutes.
3. Perform column characterization.
a. Raise the oven and injector temperatures to 50 °C and allow them to stabilize. Then
confirm that the carrier gas is on.
Note Wear clean, lint- and powder-free gloves when you handle the column and
injector ferrule.
Tip Slide a septum on the column before the injector nut to make it easier to
measure the proper distance between the nut and end of the column.
CAUTION - INSTRUMENT DAMAGE: Do not allow the clear plastic component to
exceed 80 °C. Otherwise, it will melt and damage the instrument.