Zeiss LSM 880, Руководство по эксплуатации

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16
Fig. 11
a) Variation of
pinhole diameter
b) Variation of
numerical aperture
d) Variation of
refractive index
c) Variation of
wavelength ( λ

)
fluorescent plane
fluorescent point
reflecting plane (mirror)
Optical slice
(NA = 1.3; n = 1.52;
λ  = 496 nm)
4.5
4.0
3.5
3.0
2.5
2.0
1.5
1.0
0.5
0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0
Pinhole diameter [AU]
FWHM [µm]
Depth resolution
(PH = 0; n = 1.52;
λ  = 496 nm)
1000
920
840
760
680
600
520
440
360
280
200
600
560
520
480
440
400
360
320
280
240
200
1 1.1 1.2 1.3 1.4
Numerical aperture
FWHM [nm]
Depth resolution
(PH = 0; NA = 1.3; n = 1.52)
488 504 520 536 552 568 584 600
Wavelength [nm]
FWHM [nm]
Depth resolution
(PH = 0; NA = 0.8;
λ
 = 496 nm)
1600
1520
1440
1360
1280
1200
1120
1040
960
880
800
1.33 1.36 1.38 1.41 1.44 1.47 1.49 1.52
Refractive index of immersion liquid
FWHM [nm]