Zeiss LSM 880, Руководство по эксплуатации

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CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
232 000000-2071-464 10/2014 V_01
Proceed as follows:
•
Make sure that the front lens of the water
immersion objective is wetted by a drop of
water. Use fluorescence-free double-distilled
water, since otherwise the immersion water will
cause background fluorescence and deteriorate
the correlation signal.
•
Choose a chamber by clicking on one well in
the
X, Y (Stage) panel of the Sample Carrier
tool.
•
Set up a beam path in the LSM
Imaging Setup
tool (Fig. 305).
Convenient settings are:
−
488 nm line at 50 % tube current and 5 %
AOTF
−
MBS T80/R20 major dichroic beam splitter
for visible light
−
Plate major dichroic beam splitter for
invisible light
−
None for side port systems, Rear for rear port
systems at the reflector position
−
Reflection check box activated
−
Appropriate spectral band in the detector
channel to detect the reflected excitation
light, for example 420 – 650 nm for 488 nm
light
Fig. 305 Beam path configuration for
finding glass reflection