Zeiss LSM 880, Руководство по эксплуатации

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Center Screen Area / Image Containers - Display and Image Analysis ZEISS
10/2014 V_01 000000-2071-464 531
In the
Ratio1
channel choose the
Ratio Type 3
formula. With Ch1-T1 and Ch1-T2 corresponding to I
P
and I
s
this formula corresponds to the formula for anisotropy r. For the factors put in the value for the G-
factor in the numerator and 2 x G in the denominator. Scale by Min/Max by checking the
Set by
min/max box and entering 0 in the Min input box and 1 in the Max input box . With a corresponding
look up table like rainbow this will produce a fasle colored anisotropy image in the Ratio1 channel.
Alternatively you can use the image calculator with source 1 (S1) being the
P track image (Ch1-T1) and
source 2 (S2) the
S
track image (Ch1-T2) with consideration of the G-factor (see Fig. 750).
Proceed as follows. In the
Processing tab select the Image calculator (Fig. 750). Press Select and click
on the desired input image. In the
Channel
pull down menu of
Input Image Image 1
select
ChX-T1
,
where X stand for 1, 2 or S. Accordingly, in the Channel pull down menu of Input Image Image 2
select
ChX-T2
.
Type in the anisotropy formula considering the G-factor of the system. Choose either Absolute Intensities
in case you have to multiply the formula with 2
n -1 to rescale to an n bit image. For example, if the color
depth was 8 bit, multiply with 2
8
-1=256-1=255. Alternatively, choose
Intensity range 0..1
, in which
case you do not multiply with an extra factor. Press Apply and choose a respective look up table to
obtain a false colored anisotropy image.