Zeiss LSM 880, Руководство по эксплуатации

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
10/2014 V_01 000000-2071-464 203
C. Bunching terms
Bunching is the phenomenon of a burst of photons during a certain time interval, the duration of which
is determined by photo-physical processes including triplet, blinking, flickering and protonation. These
terms are exponential decay functions. Formally, they look the same, only the exponential decay might be
different.
C.1 Triplet
) 1 ( ) ( t e T T G
t t t
t
t
t
−
⋅ + − =
not normalized
(7a)
)
1
1 ( ) (
t
t
t
T
e T
G
t
−
⋅
+ =
−
t
t
t
normalized
(7b)
where T
t
is the triplet fraction, that is the number of molecules undergoing triplet states and
t
t
the triplet
decay time.
T
t
and
t
t
are fitted parameters.
Triplet is based on an un-allowed intersystem crossing from the excited to the so-called triplet state. This
state lasts for 1 – 5 µs. If the electron drops back to the ground state, no photon is emitted and hence
during the triplet state the molecule is in a dark state. Triplet is indicated as a rise in the correlation
amplitude, which is indicated as a deviation from the flattening curve at shorter correlation times. If not
normalized, the triplet fraction contributes to the total number of molecules.
C.2 Blinking
) 1 ( ) (
b
e T T G
b b b
t
t
t
−
+ − =
not normalized (7c)
)
1
1 ( ) (
b
b
b
T
e T
G
b
−
⋅
+ =
−
t
t
t
normalized (7d)
where T
b
is the blinking fraction, that is the number of molecules in the dimmer state and
t
b
the blinking
decay time of the dimmer state. Note, if the blinking term is not normalized, the number of blinking
molecules will influence the total number of molecules.
T
b
and
t
b
are fitted parameters.