Zeiss LSM 880, Руководство по эксплуатации

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CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
108 000000-2071-464 10/2014 V_01
•
Select the
Scan Speed
from the 19 preset steps via slider or input box. Recommended: 8 for the first
scan. A click on the Max button sets the maximum speed for the current zoom.
−
In basic mode (
Show all
inactive), the LSM switches automatically to bi-directional scanning if high
scan speeds are selected (see also description of bi-directional scanning below).
−
The
Scan Speed
determines the
Pixel Dwell
time. In the case of different image formats, the
Pixel Dwell time is constant for the same Scan Speed, but the Scan Time is different.
−
Pixel Dwell
time of the laser beam on the pixel
−
The Scan Time duration of the acquisition for the entire frame
Speed: 1 ... 11 12 13 14 15 16 19
Zoom: 0.6 - 1.4
≤
1.8
≤
2.9
≤
3.3
≤
4.3
≤
5.4
≤
13.2
Mode: lbda./
34 ch.
lbda./
32 ch.
10-ch 10-ch 6-ch 6-ch 4-ch
* Values refer to image format 512 x 512 pixels, 8 bit data depth and a tube lens of 150 mm (LSM on the side
port of Axio Observer); other values apply for higher formats and data depth.
•
Select
8 Bit
,
12 Bit
or
16 Bit
Data Depth, i.e. 256, 4096 or 65536 gray values.
•
Select the Unidirectional or Bi-directional Scan Direction.
−
Unidirectional: The laser scans in one direction only, then moves back with beam
blanked and scans the next line.
−
Bi-directional: The laser also scans when moving backwards, i.e. the Scan Time is
halved. The online scanner calibration (OSC) will correct for nonlinear distortions of the lines in
bidirectional mode. This function (see also checkbox in
Maintain Tab
->
Options – System
Options -> Hardware ) eliminates most of the deviations of the scanmirrors at the very fast speeds.
−
The pixel shift between forward and backward movement (double
image) resulting from bi-directional scanning must be corrected. For automatic scan correction,
click on the
Auto
button. If
Auto
is off the scan
Corr X
and
Y
sliders which are indicated when
selecting bidirectional scanning. Zero° rotation requires correction in the X-direction, 90° rotation
must be corrected in the Y-direction. If the image was rotated, correction is required in both
coordinates. Correction is performed on-line in the
Continuous scan mode. The size of the shift
depends on the Scan Speed.
•
Select the Line or Frame mode for averaging.
•
Select the desired scan average method Mean or Sum .
•
Select the desired scan average from the available values
2
,
4
,
8
and
16
in the
Number
selection drop
down menu or Continuous (only for Frame average mode).
The greater the number of averages selected for Mean average Method , the better the image
quality will be; the scanning time will be prolonged accordingly.
Averaging can be performed in different ways, depending on whether the
Mean
or
Sum
method has
been activated.
If you are using the
Mean method, the image information is generated by adding up all scans pixel by
pixel and then calculating the mean value.