Zeiss LSM 880, Руководство по эксплуатации

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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5.3.2.3 Light Path for 32 Channel Module
The 32 Module consists of an array of 32 GaAsP detectors plus two side PMTs. The side PMT of the
longer wavelength region (Pmt2) can be used together with the GaAsP array detector for FCS. Up to six
autocorrelation channels can be defined by setting respectable spectral bands. Each of these channels
can be cross-correlated to itself or to any of the other activated channels (CC), but a total number of ten
cross-correlation channels is the upper limit.
Selection of dichroics and filters
From top to bottom you have the following
dichroics and filters available.
Main dichroic beam splitter (MBS):
The MBS reflects the specified laser line and allows
the resulting fluorescence spectrum to pass
through.
•
Click on the MBS icon to open a list of dichroics
(Fig. 264).
•
Select the appropriate one by a mouse click.
•
Close the list by clicking the icon again.
•
You have two major beam splitters, one for the
Visible light and one of the Invisible light.
There are no SBS nor EF filters, as a grating is used
for spreading the light into its spectrum, which is
projected onto the GaAsP array.
Press the
Adjust pinhole...
button to open the
Adjust pinhole panel. Here you can adjust the
pinhole for the respective beam path or method.
Fig. 264 Light Path tool opened for the 32
Channel Module