Zeiss LSM 880, Руководство по эксплуатации

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CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
140 000000-2071-464 10/2014 V_01
When highlighting a ROI in the list, the numerical
parameters for
Center X
,
Center Y
,
Width
and
Height in pixels will be shown in Region feature
field. In addition the ROI will be set to active in the
image.
Note, when a PALM ( HR ) image is
displayed numbers will refer to the PALM
image and not the
SWF
image, which
normally do have different pixel
resolutions.
It is possible to change the
Line width
from the
Line width drop down menu (Fig. 189).
The
Color
of the outline of a ROI can be changed
in the Color drop down menu (Fig. 190). The scroll
bar gives access to all available colors.
Color selection is only possible if either
Individual
colors or Common Color is chosen in the drop
down list of the
Color mode
.
The Color mode drop down menu allows defining
how colors will be assigned to the outline of a
region (Fig. 191).
Individual colors allow the
assignment of different colors to different regions.
If
Automatic Assignment
is chosen in the
Color
mode a color will be automatically assigned to
each region as it comes.
The choice of Color is inactive in this mode.
Common Color assigns the same color to all
regions.
The
Creation Mode
drop down menu offers three
different settings for defining ROIs (Fig. 191).
Switch to selection mode
activates the arrow
tool once the region is drawn in the image.
Multiple elements allow drawing multiple
regions of the same type in a sequence.
One
element only
restricts the number of ROIs to be
drawn in the image to one. Any new region
deletes the previous one.
The laser usually scans the entire line length, but is
limited in the Y-direction by the ROIs outline. The
Scan Time is therefore reduced. It is possible to
reduce the movement of the scanner also in the X
direction along the line by checking the box next
to Fit frame size to bounding rectangle of regions . This then corresponds to zooming in onto the
region. The frame size will be updated accordingly in addition to the scan time. This applies for image
acquisition using a ROI. This function will only be applied for the
LSM imaging
mode. For widefield
modes ( WF , SIM , LSM WF ) it will be ignored.
If a region is used to define a position for bleaching within the sample, the bleach process can be
accelerated by checking the box next to
Zoom Bleach (fast, less accurate) .
Fig. 189 Line width drop down menu
Fig. 190 Color drop down menu
Fig. 191 Color mode drop down menu