Zeiss LSM 880, Руководство по эксплуатации

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CHAPTER 1 - SYSTEM OPERATION
ZEISS Center Screen Area / Image Containers - Display and Image Analysis LSM 880
528 000000-2071-464 10/2014 V_01
6.20.1 Imaging Setup for Polarization
Imaging
In case emission polarizers (analyzers) are available,
you have a
Notch filter cascade selection button
available situated on top of the MBS button (see
Fig. 745).
Set up a multitrack configuration using exactly the
same settings with the exceptions of the analyzers.
To this end press the
Notch filter cascade button.
This opens up the
Polarizer selection box
. Check
the polarizer which you want to use with track 1.
The options are
P (for the parallel polarized light
transmission) and S (for vertical polarized light
transmission) (see Fig. 746).
If you want to ratio image, then it is best for
convenience to choose
P for track 1. Check the
other polarizer for track 2.
For ratio imaging this would be
S
. For ratio
imaging check the R1 channel box and assign
Source 1
to the selected channel-T1 and
Source 2
to the selected channel-T2. You can use the single
PMTs
Ch1 and Ch2 or the spectral detector ChS .
Fig. 745 Imaging Setup with Notch filter
control button
Fig. 746 Imaging Setup with Notch filter
cascade option