Zeiss LSM 880, Руководство по эксплуатации

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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If a region is used to define a position for bleaching within the sample, the bleach process can be
accelerated by checking the box next to
Zoom Bleach (fast, less accurate)
. In this case the scanner
movement will be restricted to the bleach region zooming in onto this region. This may result in a less
accurate positioning of the region as the definition of the region has been made in a different zoom in
the image. The bleach process will be faster than without this option. The gain in speed is dependent on
the speed that is used for bleaching. If a high speed has already been chosen for bleaching the gain in
speed will be minimal.
Define the region(s) for bleaching in the
Regions tool (see section Acquisition Parameter – Regions ).
Only a region activated under Bleach is considered for bleaching. In the
Excitation of Bleach
area
(Fig. 202) choose the laser line and laser intensity which is then used for bleaching.
It is possible to choose different laser lines and/or laser intensities for the bleach process of each region
when activating the option:
Use different Settings for different ROIs
. A maximum number of six
regions is possible. The numbers of the regions correspond to the numbers indicated next to ROI# . For
each region, a tab is active which allows setting individual laser parameters for bleaching.
Define the region(s) for bleaching in the
Regions
tool. Only a region activated under Bleach is
considered for bleaching. In the
Excitation of
Bleach area (Fig. 203) chose the laser line and
laser intensity which is then used for bleaching.
It is possible to choose different laser lines and/or
laser intensities for the bleach process of each
region when activating the option:
Use different
Settings for different ROIs
. The numbers of the
regions correspond to the numbers indicated next
to
ROI# . For each region, a tab is active which
allows setting individual laser parameters for
bleaching.
The
Regions tool is only available in the LSM
imaging mode.
Fig. 203 Excitation of Bleach area for setting
laser line and intensity for
bleaching