Zeiss LSM 880, Руководство по эксплуатации

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CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
58 000000-2071-464 10/2014 V_01
(1) Conventional setting of the microscope
Axio Observer.Z1
The Recording of microscope settings works as
described for the microscope Axio Imager.
For the conventional setting of the
Axio Observer.Z1, proceed as follows:
•
Click the
Locate
tab in the
Main
toolbar.
•
Place specimen on microscope stage.
−
The cover slip must be facing down.
•
In the Objective list box, select the required
objective.
•
Use the focusing drive (Fig. 59/ 4 ) to focus the
required specimen plane.
•
Select specimen detail by moving the stage in
X and Y via the XY stage fine motion control
(Fig. 59/
3 and 2 ).
(2) Microscope settings on Axio Observer for transmitted-light observation
•
Click on the
Transmitted light
button. Click the
On
button in the
Transmitted Light
panel and set
the transmitted light intensity via the slider or click on 3200 K .
•
Click on the Condenser button and set the aperture via the slider in the Condenser panel. Set the
filter in the
Filter
selection box.
•
Click on the Objective button and select the objective by clicking on it.
•
Click on the Reflector button and select None .
•
Make sure there is no illumination from the reflected-light.
Fig. 58 Axio Observer.Z1 controls in the
Microscope Control tool