Zeiss LSM 880, Руководство по эксплуатации

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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When in
Channel
mode the image acquisition can
either be done in one Track (all lasers and
detectors active simultaneously) or in up to 4 tracks
(Fig. 97).
Configurations for each track are thereby defined
in the
Beam path configuration
field for the
selected track. To create a new track configuration,
highlight the track. Go to the
Beam path
configuration to set up the beam path. You can
store the track configuration in the
Experiment
Manager field. The configuration also includes the
settings of laser power, detector gain and offset to
be selected in the Channels tool window (see
section 5.2.6).
Tracks can be added or deleted by pressing the
+
or
dust bin
button, respectively. The
dust bin
button is inactive if only on track is defined. Up to
4 tracks can be added. The
+ button becomes
inactive after the 4
th
track has been added. An
added track will have the same filter settings than
the first track, which can be subsequently changed
in the
Beam path configuration field. The Track
button of the selected Track (1-4) will be
highlighted in blue and the Track display area
below designates the track that is displayed in the
Beam path configuration field. All alterations
will be specific for that track.
If all channels of a track are deactivated
this track will be deactivated, too.
With the Switch track every drop down menu
(only available if
Show all
is active), you can select
the way tracks are switched. The different tracks
can be switched every
Line
(available for
LSM
imaging mode only), every Frame , every Frame
Fast
and every
Z-Stack
(if Z-Stack acquisition is
checked) (Fig. 98).
In
Line and Frame Fast mode, switching of hardware components that require mechanical movement
like filter change is prevented and hence the setting of the first track remains in place, even if the other
tracks have different configurations.
The latter parameters are therefore overwritten by those from the first track. Only those settings that do
not require mechanical movement are accepted like activation/deactivation of laser lines and channels,
and laser power settings. You will be prompted in an Info window to allow parameters to be overwritten
when using
Line
and
Frame Fast
switching modes (Fig. 99). Press
Yes
to accept parameters to be
overwritten. Press No to return to Frame or Z-Stack switching mode, where all settings will be kept for
each track. Note, that if mechanical switching is required, switching time will be accordingly slow.
The total set of parameters for one or multiple tracks can be stored as the active acquisition
parameters using the save option of the
Experiment Manager field. Using multiple tracks is
the preferred method to clearly separate weakly overlapping emission signals.
Fig. 97 Track definition
Fig. 98 Switch track every drop down
menu
Fig. 99 Info window to confirm