Zeiss LSM 880, Руководство по эксплуатации

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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5.4.22.3 Channel Shift
Channel shift is used to produce a congruent
image with relation to the pixels of the various
channels (Fig. 550).
This pixel correction function is particularly
important in UV applications.
This is a manipulation of your data that has
to be considered very carefully when co-
localization analysis is part of your
experiment.
The
Select button selects the active image in the
Image Display
as the Input Image.
The image has to be a multi-channel image.
•
Select the channels you want to shift by ticking
the Ch1-T1 or Ch2-T2 check box:
•
Use the X and Y sliders or spin boxes to define
the shift in pixels to select the pixel shift in the
horizontal and vertical direction. Clicking the
Zero next to the spin-boxes resets the shift to
the original position.
A preview is automatically generated at the
bottom of the
Channel Shift
tool in the
Processing tab of the Left Tool Area At the same
time, a large preview is shown in the
Preview
view tab in the Image display in the Center Screen
Area.
•
Click on the Apply button to generate a new
image with the set pixel shift.
Fig. 550 Processing – Adjust with Channel
shift