Waters Xevo G2 QTof, Operator'S, Overview And Maintenance Manual, Page: 75 / 248

Optional glass capillary sprayer 4-13
6. Load sample into the capillary using either a fused silica syringe needle
or a GELoader tip.
Tip:
Shake the loaded capillary to move the liquid to the tip of the
sprayer.
7. With the sprayer mounted on the adjuster platform, screw the union
back into the assembly; finger-tight is sufficient.
8. In the console software, ensure the capillary parameter is set to 0 V.
9. Push the sprayer platform into the source (see page 4-6 ).
To optimize the glass capillary sprayer:
1. Set the NanoFlow gas pressure to 0.3 bar and the cone gas to 40 L/h.
2. Ascertain that sample is flowing by observing a droplet on the tip.
Tip:
If you cannot observe a droplet, increase the pressure briefly, up to
a maximum of 1.5 bar, and then return the pressure to 0.3 bar.
3. If you observe a droplet, continue the procedure at step 9 .
Requirement:
If you do not observe a droplet, in the console software,
ensure the capillary parameter is set to 0 V, and follow step 4 through
step 8 .
4. Move the sprayer back and to the left-hand side until the tip aligns with
the groove on the cone.
Tip:
Aligning is best done while viewing from the front of the source.
Warning:
To avoid eye injuries, always wear eye protection when cutting
fused silica.
Warning:
To avoid injury from trace chemicals adhering to the probe,
always wear chemical-resistant, powder-free gloves.