Thermo Scientific
imPULSE S.U.M. with Touchscreen Console User’s Guide
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134
Chapter 6
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Operating information
6.6.7 Sampling
Sampling with a recirculation loop
Samples can be taken from the SmartSite port on the recirculation
loop by attaching a luer lock syringe and drawing a sample while the
recirculation loop is operating. Samples can also be taken on BPCs
equipped with a thermowell/sampling port located in one of the probe
ports. The following instructions are for aseptic sampling via the
SmartSite port. For these instructions, use the standard luer lock on a
60 mL syringe, or sterile manifold.
1. Remove the dust cover from the SmartSite (Figure 6.5).
2. Clean the SmartSite with a sanitary wipe. Then, connect the
sanitary luer lock type syringe (Figure 6.6).
Figure 6.5. Removing the
cap.
Figure 6.6. Connecting
sampling syringe.
3. Apply a small amount of vacuum pressure by pulling out the syringe
plunger slightly and pull a sample (approx. 30–60 mL).
4. Remove the syringe. This will be considered a purge sample.
5. Clean the SmartSite with a sanitary wipe. Then, connect the
sanitary luer lock type syringe.
6. Apply a small amount of vacuum pressure using the syringe, and
pull the desired sample volume (approx. 10–20 mL).
7. Remove the syringe. This will be considered a representative
sample.
8. Clean the SmartSite with a sanitary wipe. Then replace the dust
cap.
