Thermo Scientific Dionex ICS-2000 Operator'S Manual Download Page 5

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Contents

Doc. 031857-03 4/06

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3.10.1 Loading Samples with a Syringe . . . . . . . . . . . . . . . . . . . . . .3-15

3.10.2 Loading Samples with a Vacuum Syringe . . . . . . . . . . . . . . .3-16

3.10.3 Loading Samples with an Autosampler . . . . . . . . . . . . . . . . .3-16

3.10.4 Injecting Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-17

3.10.5 Example Chromeleon Commands for Loading and

Injecting Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-17

3.11

Process Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-18

3.11.1 Manual Sample Processing . . . . . . . . . . . . . . . . . . . . . . . . . .3-18

3.11.2 Automatic (Batch) Sample Processing . . . . . . . . . . . . . . . . . .3-19

3.12

Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-21

4 • Troubleshooting

4.3

Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

4.1

Troubleshooting Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3

4.2

Liquid Leaks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-12

4.3

Pump Difficult to Prime or Loses Prime . . . . . . . . . . . . . . . . . . . . . . .4-14

4.4

Pump Does Not Start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-16

4.5

No Flow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-17

4.6

Erratic Flow/Pressure Reading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-18

4.7

Excessive System Backpressure . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-18

4.8

Peak “Ghosting” . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-19

4.9

Nonreproducible Peak Height or Retention Time . . . . . . . . . . . . . . . .4-20

4.10

Abnormal Retention Time or Selectivity . . . . . . . . . . . . . . . . . . . . . .4-20

4.11

No Cell Response . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-20

4.12

High Cell Output . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-21

Summary of Contents for Dionex ICS-2000

Page 1: ...ICS 2000 ION CHROMATOGRAPHY SYSTEM OPERATOR S MANUAL Document No 031857 Revision 03 April 2006...

Page 2: ...TATIONS REGARDING THE USE OR THE RESULTS OF THE USE OF THIS PUBLICATION IN TERMS OF CORRECTNESS ACCURACY RELIABILITY CURRENTNESS OR OTHERWISE FURTHER DIONEX CORPORATION RESERVES THE RIGHT TO REVISE TH...

Page 3: ...ty Labels 1 8 2 Features 2 1 Operating Features 2 1 2 1 1 Front Panel 2 1 2 1 2 Top Cover 2 6 2 1 3 Component Panel 2 7 2 1 4 Rear Panel 2 10 2 2 The Chromeleon Interface 2 12 2 2 1 The Chromeleon Mai...

Page 4: ...eleon 3 4 3 4 Set Up the Eluent Reservoir 3 6 3 4 1 Filter the Deionized Water 3 6 3 4 2 Fill the Reservoir 3 7 3 4 3 Set the Eluent Level 3 7 3 4 4 Connect the Reservoir 3 9 3 5 Check All Connections...

Page 5: ...ing 3 18 3 11 2 Automatic Batch Sample Processing 3 19 3 12 Maintenance 3 21 4 Troubleshooting 4 3 Error Messages 4 1 4 1 Troubleshooting Error Messages 4 3 4 2 Liquid Leaks 4 12 4 3 Pump Difficult to...

Page 6: ...2 Isolating a Restriction in the Liquid Lines 5 10 5 3 Replacing Tubing and Fittings 5 14 5 4 Rebuilding the Injection Valve 5 15 5 5 Cleaning and Replacing the Pump Check Valves 5 16 5 6 Replacing a...

Page 7: ...and Conditioning the EPM 5 68 5 20 Replacing the EGC CO3 Mixer 5 69 5 20 1 Installing the New EGC CO3 Mixer 5 69 5 20 2 Filling the EGC CO3 Mixer with Deionized Water 5 70 5 20 3 Filling the EGC CO3 M...

Page 8: ...on B 1 Facility Requirements B 1 B 2 Unpacking the ICS 2000 System B 2 B 2 1 Unpacking the Computer North America only B 4 B 2 2 Unpacking the Computer outside North America B 4 B 3 Installing Chromel...

Page 9: ...ng the Pump B 31 B 13 1 Priming the Eluent Lines with a Syringe B 32 B 13 2 Priming the Pump Heads with the Prime Button B 34 B 14 Setting Up the Eluent Generator B 35 B 14 1 Eluent Generator Setup fo...

Page 10: ...tions Optional B 116 B 23 Manually Connecting to a Control Panel B 118 B 24 Installation Troubleshooting B 120 C Touch Screen Operation C 1 Using the Touch Screen C 1 C 2 Using the Touch Screen with C...

Page 11: ...3 Controlling the Eluent Generator Power C 21 C 8 4 Monitoring the EluGen Cartridge Life C 21 C 8 5 Controlling the CR TC Power C 22 C 9 Suppressor Page C 23 C 10 Detector Page C 24 C 10 1 Setting th...

Page 12: ...etention times moving F 1 F 4 How do I adjust retention times F 1 F 5 When should I remake standards F 1 F 6 When should I replace the eluent generator cartridge F 2 F 7 How do I start Chromeleon F 2...

Page 13: ...suppressor a conductivity cell and a data collection system Before running a sample the ion chromatography system is calibrated using a standard solution By comparing the data obtained from a sample...

Page 14: ...nd concentration remain constant throughout the run Gradient delivery a change in concentration over time is possible when the ICS 2000 is controlled by Chromeleon Chromatography Management System the...

Page 15: ...they flow through a suppressor that selectively enhances detection of the sample ions while suppressing the conductivity of the eluent 5 Detection A conductivity cell measures the electrical conductan...

Page 16: ...running Microsoft Windows XP or Windows 2000 and Chromeleon software version 6 5 SP2 or later Chromeleon also provides data acquisition and data processing functions Locally with the front panel LCD t...

Page 17: ...rmation Chapter 2 Features Overview Provides an overview of ICS 2000 operating features and system components introduces the Chromeleon user interface Chapter 3 Operation and Maintenance Provides oper...

Page 18: ...ol Describes the ICS 2000 TTL and relay control features Appendix E Reordering Information Lists spare parts for the ICS 2000 Appendix F FAQ Provides answers to frequently asked questions about ICS 20...

Page 19: ...t e pourrait entra ner des blessures graves mortelles Signale une situation de danger potentiel qui si elle n est pas vit e pourrait entra ner des blessures mineures mod r es galement utilis pour sign...

Page 20: ...in compliance with the following standards EN 61010 1 2001 safety CAN CSA C22 2 No 1010 1 92 A2 97 safety UL 3101 1 10 93 safety and EN 61326 1997 including A1 1998 and A2 2001 EMC susceptibility and...

Page 21: ...or automated sample injection the ICS 2000 must be connected to an autosampler For more information about sample injection see Section 3 10 Touch Screen The LCD touch screen provides local control of...

Page 22: ...hile command buttons are rounded To select a command button or edit a field touch and release the command button or edit field with your fingertip NOTE When you touch a button or edit field the action...

Page 23: ...field for example opens a page with a number keypad see Figure 2 3 Use the keypad to enter the desired numerical value for the field and then touch the ENTER button Figure 2 3 Number Keypad Touch a n...

Page 24: ...me for example in the bottom right corner opens a menu of pages see Figure 2 4 Touching a page name on the menu of pages displays the selected page For example touching SUPPRESSOR displays the Figure...

Page 25: ...ls about each touch screen page Touching returns you to the HOME page Touching opens a list of options see Figure 2 6 Figure 2 5 Suppressor Page Figure 2 6 Suppressor Page Selecting an Option Touch to...

Page 26: ...View EGC EPM and CR TC Connectors The cable from the EluGen cartridge EGC connects to the EGC 1 connector The cable from the Continuously Regenerated Trap Column CR TC connects to the CR TC connector...

Page 27: ...ws the user accessible components installed on the component panel behind the ICS 2000 front door Figure 2 8 ICS 2000 Component Panel Mounting Brackets for a Second Suppressor DS6 Cell Injection Valve...

Page 28: ...low rate to 0 00 mL min turns off the pump See Section 2 4 3 for details about the pump Pressure Transducer The pressure transducer measures the system backpressure Injection Valve The injection valve...

Page 29: ...h conductivity cell measures the electrical conductance of analyte ions as they pass through the cell A heat exchanger inside the cell regulates the temperature which can be set to between 30 and 55 C...

Page 30: ...r or recording device See Section B 20 for connection and setup information USB Connectors A USB receptacle is provided to allow connection to the Chromeleon computer Two USB ports are provided for co...

Page 31: ...bing routed from the top cover in place Power Switch The power switch provides on off control of power to the ICS 2000 Main Power Receptacle The power supply cord plugs into the AC power receptacle Th...

Page 32: ...eon menu bar toolbars and Control panels Direct control commands are executed as soon as they are entered Section 2 2 2 describes the commands available from the ICS 2000 Control panels With automated...

Page 33: ...nels for the ICS 2000 System Control panels are used for controlling and monitoring ICS 2000 operation System Control panels are described below The Wellness Panel is used for performing calibration a...

Page 34: ...ICS 2000 Ion Chromatography System 2 14 Doc 031857 03 4 06 Figure 2 11 ICS 2000 System Control Panel Traditional Figure 2 12 ICS 2000 System Control Panel Graphical...

Page 35: ...the database in which the chromatographic data for the sample is saved Sequence Displays the sequence in which this sample is included Sample Displays the name of the sample that is running Program D...

Page 36: ...nd the module Clearing the check box disconnects the ICS 2000 from the PC Startup Starts the pump suppressor and eluent generator The flow rate suppressor current and eluent concentration settings tha...

Page 37: ...ls Off On Prime Turn the pump on and off or start the pump priming function Eluent Flow Valve Select the eluent flow valve position Closed or Open Max Pressure Displays the upper pressure limit If thi...

Page 38: ...the injection valve Load Switches the valve to the load position Inject Switches the valve to the inject position Injection Valve Switch Switches the valve from the current position to the other posit...

Page 39: ...total conductivity is the background conductivity the eluent conductivity before sample injection Touch Autozero to zero the conductivity and offset this background reading from the total reading Sign...

Page 40: ...ck to select plot options from a context menu You can select autoscale commands change the plot appearance or select other signals to be displayed for example total conductivity or pump pressure Conce...

Page 41: ...impaired until a new cartridge is installed Cartridge Type Displays the type of cartridge installed Remaining Life Displays the percentage of ions remaining in the cartridge The ion percentage is cou...

Page 42: ...tion to the concentration of K2CO3 required for your application and set the EGC 2 Target Concentration to the concentration of KHCO3 required Applied Concentration Displays the concentration of eluen...

Page 43: ...required components include the corresponding type of EluGen cartridge and a CR TC Figure 2 14 illustrates the liquid flow path when the components required for producing carbonate bicarbonate eluent...

Page 44: ...hanger Guard Degas Out Pulse Damper EGC Degas From the pulse damper Regen In Regen Out Eluent Out Eluent Degas In To waste Press Xducer Eluent Valve Pump Head Pump Head Separator CR TC 2 3 4 5 7 8 12...

Page 45: ...u and on to the injection valvev After sample is loaded into the sample loopw and the injection valve is toggled to the Inject position eluent passes through the sample loop The eluent sample mixture...

Page 46: ...s In Eluent In Regen In Regen Out Eluent Out Cell In Cell Out Column Heater Suppressor Heat Exchanger Guard Degas Out Pulse Damper Valve 11 EGC Degas Degas In To waste Eluent Valve Separator 2 3 4 5 8...

Page 47: ...u to the EGC CO3 Mixerv to ensure a homogenous carbonate bicarbonate mixture and then on to the injection valvew After sample is loaded into the sample loop and the injection valve is toggled to the I...

Page 48: ...n Regen Out Eluent Out Cell In Cell Out Column Heater Suppressor Heat Exchanger Guard Degas Out Pulse Damper EGC Degas Degas In To waste Eluent Valve Separator 2 3 4 5 6 EGC II K2CO3 Inlet Outlet To G...

Page 49: ...genous eluent concentration and then on to the injection valvev After sample is loaded into the sample loopw and the injection valve is toggled to the Inject position eluent passes through the sample...

Page 50: ...ed in the ICS 2000 at the factory consists of A single channel degas chamber with degas membranes with internal capacity of 17 mL A dual stage diaphragm vacuum pump A solenoid valve An on board vacuum...

Page 51: ...ump is always on This setting is for test purposes by a Dionex Service Representative Do not use this setting for routine operation Cycle The degas pump cycles on and off according to the times specif...

Page 52: ...h prevent reverse flow through the pump are located on the bottom inlet and top outlet of the primary pump head The priming valve is on the front of the pump head The eluent valve controls the flow fr...

Page 53: ...ad delivers eluent to the remainder of the chromatography system the injection valve column and detector The waste valve is located on the front of the secondary pump head see Figure 2 18 To open the...

Page 54: ...rol program or from the touch screen PUMP page see Section C 7 See Section 4 1 for troubleshooting information if a pressure limit is exceeded Pulse Damper Flow output from the pressure transducer con...

Page 55: ...generation For more information refer to the EluGen cartridge manual The manual is included on the Dionex Reference Library CD ROM P N 053891 EluGen Cartridge Part Number Function EGC II K2CO3 EluGen...

Page 56: ...en cartridge and an EPM that perform as one cartridge Note that the allowable eluent concentration for a linked cartridge is less than when the cartridge is defined as independent For EluGen Cartridge...

Page 57: ...ic pH Modifier 0 1 to 15 mM at 0 1 to 1 0 mL min flow 0 1 to X mM at 1 0 to 2 0 mL min flow X 15 flow The total of the eluent concentrations from both the EluGen cartridge and the EPM must not exceed...

Page 58: ...on valve and the EluGen cartridge OUTLET port For details refer to Section B 16 1 Continuously Regenerated Trap Column CR TC The CR TC is a high pressure electrolytically regenerated trap column The C...

Page 59: ...K2CO3 and KHCO3 eluent For more information about these products refer to the EluGen cartridge manual Cartridge manuals are included on the Dionex Reference Library CD ROM P N 053891 Generating Carbon...

Page 60: ...automated sampler line if installed through the valve and into the sample loop Excess sample flows out to waste In the Inject position sample is swept to the column for analysis Eluent flows from the...

Page 61: ...4 MPa 2000 psi of system backpressure for removal of electrolysis gas from the eluent produced by the cartridge A system backpressure of 16 MPa 2300 psi is ideal If the system backpressure is too low...

Page 62: ...ressor manuals The manuals are on the Dionex Reference Library CD ROM P N 053891 The column heater provides temperature control for the separator and guard column The heater temperature can be set to...

Page 63: ...f temperature variation is minimized by suppressing eluent conductivity To further reduce the effect of temperature variation a heater inside the cell regulates the ttemperature The cell heater can be...

Page 64: ...ICS 2000 Ion Chromatography System 2 44 Doc 031857 03 4 06...

Page 65: ...r any other purpose Operation of the ICS 2000 in a manner not specified by Dionex may result in personal injury 3 1 Operation Overview Figure 3 1 illustrates the basic steps for routine operation of t...

Page 66: ...Manual Sample Processing Using the Front Panel A third party recording device must be connected to the analog output Optional Record data from the analog output Inject the sample Autozero Manual Samp...

Page 67: ...on the power to the computer and the autosampler if used Figure 3 3 ICS 2000 Rear Panel Feature Power Up Condition Pump Off Injection valve Load position Cell Reading current value Suppressor Off Cell...

Page 68: ...not running the icon on the taskbar is crossed out in red start the Server by right clicking the icon and selecting Start Server When the server starts the icon changes to gray 2 Start Chromeleon by...

Page 69: ...than the traditional style and include more controls You can choose either style of panel depending on your personal preference 4 Open a panel for your system configuration by double clicking its name...

Page 70: ...s in the deionized water that may contaminate the pump check valves and cause erratic flow rates or loss of prime An end line filter P N 045987 is provided in the ICS 2000 Ship Kit P N 064375 for this...

Page 71: ...e ICS 2000 updates the field as the eluent is used up A warning first appears if the level falls below 200 mL Warnings are repeated at 100 mL and 0 mL In order for the eluent level displayed on the sc...

Page 72: ...3 4 06 The STATUS page see Figure 3 9 also displays the amount of liquid remaining in the reservoir and an estimate of how many hours remain before the eluent reservoir is empty See Section C 6 for de...

Page 73: ...reservoir is filled and the tubing connecting the reservoir to the ICS 2000 tubing is securely connected see Figure 3 10 2 Make sure the blue eluent generator electrical cable is connected to the EGC...

Page 74: ...onfirm that the waste valve is open by pressing OK when the reminder message appears The pump will begin pumping at approximately 3 mL min 4 Continue priming the ICS 2000 until all air and previous el...

Page 75: ...current and eluent concentration settings that were in effect when the system was shut down are restored 1 Verify that the pump is on and set to the correct flow rate 2 Verify that the suppressor curr...

Page 76: ...ure for the installed column refer to the column manual for details and is stable If the pressure is less than the expected amount gas may be trapped in the system To release the gas remove the pump f...

Page 77: ...clean containers with strong acids or detergents because these can leave traces of ions on the container walls The ions may interfere with the analysis If samples will not be analyzed on the day they...

Page 78: ...ple When using carbonate eluents diluting with eluent minimizes the effect of the water dip at the beginning of the chromatogram If you dilute the sample with eluent also use eluent from the same lot...

Page 79: ...Chromeleon timebase see Section B 18 2 An injection valve should not be installed in the AS or AS50 USB See Section B 18 for AS or AS50 USB autosampler connection instructions NOTE The ICS 2000 and t...

Page 80: ...e the free end of the line into the sample 3 Verify that the injection valve is in the Load position 4 Insert the 1 cc syringe P N 016388 provided in the ICS 2000 Ship Kit P N 064375 into the injectio...

Page 81: ...the Inject button on the HOME page Automatically Include the Inject command in a Chromeleon program See the examples in Section 3 10 5 3 10 5 Example Chromeleon Commands for Loading and Injecting Sam...

Page 82: ...les manually select operating parameters and commands from the touch screen pages or from the Chromeleon menu bar toolbars and Control panel Commands are executed as soon as they are entered NOTE When...

Page 83: ...urce This data is overwritten each time a new manual sample is processed To save the data from a manual run select the MANUAL folder select File Save As and enter a new name for the sequence 7 If you...

Page 84: ...f the steps click the Help button on the Sequence Wizard page NOTE A simple way to get started is to select the Use Application Template option in Step 4 of the Sequence Wizard This option lets you se...

Page 85: ...t 3 12 Maintenance This section describes routine maintenance procedures that users may perform All other maintenance procedures must be performed by Dionex personnel As Needed Regularly check the elu...

Page 86: ...weekly or whenever it becomes discolored Replace the filter more often if bacterial buildup is visible or if the eluent does not contain solvent NOTE It is especially important to regularly replace e...

Page 87: ...leware the instrument control firmware installed in each ICS 2000 module periodically checks the status of certain parameters If a problem is detected it is displayed on the touch screen Error message...

Page 88: ...age 5 EGC 1 disconnected error page 5 EGC 1 invalid concentration vs flow rate error page 6 EGC 1 invalid flow rate error page 6 EGC 1 over current page 7 EGC 1 over voltage page 7 EGC 2 calibration e...

Page 89: ...olumn heater is unplugged from the component panel To troubleshoot 1 Check that the column heater is plugged into the component panel 2 If the error persists the column heater may be faulty Replace th...

Page 90: ...error persists contact Dionex for assistance There may be a malfunction in the CR TC control electronics This message appears when you click the Shutdown button on the Chromeleon Control panel The mes...

Page 91: ...ponents cannot be serviced by the user This error occurs if the measured current is outside the expected range when running the eluent generator calibration procedure To troubleshoot Review the proced...

Page 92: ...to the column manual which is included on the Dionex Reference Library CD ROM P N 053891 for the concentration of eluent to use with your application Reset the eluent concentration and or the flow rat...

Page 93: ...e EGC current is turned off to prevent damage to the EGC This error may occur if the liquid flow to the cartridge is interrupted To troubleshoot See Section 4 5 for troubleshooting when there is no fl...

Page 94: ...the ICS 2000 Moduleware reports an error to Chromeleon and the Load Inject valve error message appears To troubleshoot 1 If a sequence is being executed terminate the sequence by selecting Batch Stop...

Page 95: ...istance The ICS 2000 electronics components cannot be serviced by the user If the system pressure exceeds the set limit for at least 0 5 seconds this error message appears and the pump stops Pressure...

Page 96: ...leaks see Section 4 2 3 Check that the waste valve is closed 4 Prime the pump see Section B 13 5 Restart the pump This error may be caused by an expended or dirty suppressor or by a malfunction in the...

Page 97: ...low rate Rehydrate the suppressor Refer to the suppressor manual for instructions Suppressor manuals are included on the Dionex Reference Library CD ROM P N 053891 If the error persists replace the su...

Page 98: ...g requirements The manual is on the Dionex Reference Library CD ROM P N 053891 Broken liquid line Replace the line and fittings with the same length and internal diameter tubing see Section 5 3 Blocke...

Page 99: ...ansducer cannot be serviced by the user Leaking pump head waste valve Make sure the waste valve is closed To close the valve turn the knob clockwise and tighten fingertight DO NOT OVERTIGHTEN Overtigh...

Page 100: ...problem continues contact Dionex for assistance 4 3 Pump Difficult to Prime or Loses Prime Excessive pressure fluctuations more than 3 difference from one pressure reading to the next indicate that th...

Page 101: ...me On to 30 seconds and the time Off to 10 minutes If Cycle is already selected try increasing the time on or decreasing the time off NOTE Degas Mode options can also be selected from the touch screen...

Page 102: ...to tighten the two acorn nuts that attach the pump head to the pump housing see Figure 5 11 Tighten the nuts evenly 12 in lb torque If the leak persists replace the piston seal see Section 5 6 Liquid...

Page 103: ...l fingertight DO NOT OVERTIGHTEN Overtightening may damage the valve and the pump head Flow rate is set to 0 Set the flow rate from the Chromeleon Control panel or from the touch screen HOME page Elue...

Page 104: ...ening requirements The manual is on the Dionex Reference Library CD ROM P N 053891 4 7 Excessive System Backpressure Restriction in the system plumbing Check all liquid lines for crimping or blockage...

Page 105: ...a malfunctioning injection valve These peaks may co elute with peaks of interest resulting in nonreproducible peak heights areas Insufficient time between sample injections Wait until the previous sa...

Page 106: ...t eluent Check the EGC concentration setting Contaminated or degraded sample Take appropriate precautions when preparing and storing samples to prevent contamination and degradation see Section 3 9 Co...

Page 107: ...Output Background not suppressed by suppressor Verify that the suppressor is turned on and the current is set to the correct value Refer to the suppressor manual for additional troubleshooting guidanc...

Page 108: ...ent Check the EGC concentration setting Inadequate system backpressure Add backpressure tubing between the injection valve and the ELUENT OUT port on the EGC to maintain a pressure of 16 0 7 MPa 2300...

Page 109: ...Does Not Run Degas option not enabled in Chromeleon 1 Open the Chromeleon Server Configuration program Select the ICS 2000 device icon under the timebase and select Edit Properties or double click th...

Page 110: ...selecting the Always On option The pump should turn on immediately If it does not the vacuum degas assembly may need to be replaced Contact Dionex for assistance NOTE Degas Mode options can also be se...

Page 111: ...on Procedures Diagnostic and calibration procedures can be performed either from the touch screen or from the Chromeleon Wellness Panel see Figure 5 1 For an overview of the Wellness Panel features se...

Page 112: ...lness Panel 1 Open the Chromeleon Browser and expand the Dionex Templates folder 2 Expand the Panels folder and then the Wellness folder 3 In the right pane of the Browser window double click ICS 1000...

Page 113: ...l electronics using a dummy cell Upload calibration values from the ICS 2000 to the Chromeleon Wellness database After completing a calibration procedure from the touch screen always update the Chrome...

Page 114: ...d calibration pages NOTE The Service button provides access to service functions that are performed only by Dionex personnel An access code is required to go to the service pages NOTE The Diagnostic t...

Page 115: ...ell and calibrate it 1 Open the ICS 2000 Wellness Panel in Chromeleon see Section 5 1 1 or open the touch screen ELECTRIC CONDUCTIVITY CALIBRATION page see Figure 5 3 Items Needed Description 1 0 mM K...

Page 116: ...n valve 6 Disconnect the line from the suppressor ELUENT OUT port to the cell inlet and connect the pump output line directly to the cell inlet 7 Verify that the backpressure is at least 7 MPa 1000 ps...

Page 117: ...libration values in the Chromeleon Wellness database To do this open the Wellness Panel see Section 5 1 1 and click Upload under Update Wellness Database 14 Flush the KCl solution from the system by p...

Page 118: ...touch Reset Cal on the ICS 2000 touch screen 4 Select 1 mL min to set the flow rate and begin pumping deionized water 5 Allow the pump to stabilize for 20 minutes 6 If you are using the Wellness Panel...

Page 119: ...Wellness database To do this open the Wellness Panel see Section 5 1 1 and click Upload 5 1 5 Calibrating the Vacuum Degas Assembly When to Calibrate After every 6 months of use 1 Open the ICS 2000 We...

Page 120: ...ystem backpressure 1 Begin pumping eluent through the system including the columns 2 Follow the ICS 2000 flow schematics in Figure 5 7 Figure 5 8 or Figure 5 9 and work backward through the system beg...

Page 121: ...ssor Heat Exchanger Guard Degas Out Pulse Damper EGC Degas From the pulse damper Regen In Regen Out Eluent Out Eluent Degas In To waste Press Xducer Eluent Valve Pump Head Pump Head Separator CR TC 2...

Page 122: ...Out Eluent Out Cell In Cell Out Column Heater Suppressor Heat Exchanger Guard Degas Out Pulse Damper Valve 11 EGC Degas Degas In To waste Eluent Valve Separator 2 3 4 5 8 6 EGC II K2CO3 Inlet Outlet T...

Page 123: ...umn Heater Suppressor Heat Exchanger Guard Degas Out Pulse Damper EGC Degas Degas In To waste Eluent Valve Separator 2 3 4 5 6 EGC II K2 CO3 Inlet Outlet To Gas Separator Assembly Conductivity Cell Sa...

Page 124: ...used for connections to the suppressor REGEN OUT port and the eluent reservoir Tubing Size and Type Color Used For 0 125 mm 0 005 in ID PEEK P N 044221 Red Connection from pump pulse damper to pressur...

Page 125: ...p from the ICS 2000 Control panel in Chromeleon or from the touch screen HOME page 2 Open the ICS 2000 front door 3 Disconnect each liquid line connected to the injection valve 4 Follow the instructio...

Page 126: ...e touch screen PUMP page 2 Turn off the main power switch to ensure that you do not unintentionally start the ICS 2000 3 To prevent contamination of pump parts put on a pair of rubber gloves before di...

Page 127: ...itional one quarter to one half turn 8 Reconnect the liquid lines Turn on the ICS 2000 main power 9 Open the eluent valve from the ICS 2000 Control panel in Chromeleon or select Eluent Valve Closed on...

Page 128: ...age 4 To prevent contamination of pump parts put on a pair of rubber gloves before disassembling the pump head Removing the Head and Piston 1 Turn off the main power switch to ensure that you do not u...

Page 129: ...70 Primary Pump Head P N 055711 Seal Guide P N 058032 Spacer with Drain P N 057913 Priming Valve Knob P N 055709 Inlet Check Valve Assembly P N 045722 Outlet Check Valve Assembly P N 045721 O Ring P N...

Page 130: ...nd of the piston shaft and remove the piston from the guide 3 Hold the new piston rinse seal P N 048722 with the grooved side facing up 4 Using your fingertip gently press the piston rinse seal into t...

Page 131: ...he piston seal is not removed make sure the 10 32 fitting plug s are tight and add more water Make sure the head contains no air bubbles and then repeat Steps 2 and 5 6 Remove the 10 32 fitting plug s...

Page 132: ...et in the follower picks up the piston The follower is the cylinder that holds the piston in place as it moves in and out of the pump head assembly 3 Reinstall the head and piston assembly use a wrenc...

Page 133: ...n Chromeleon or select Eluent Valve Closed on the touch screen PUMP page 2 Turn off the main power switch to ensure that you do not unintentionally start the ICS 2000 3 To remove the waste valve or pr...

Page 134: ...Slide a new O ring P N 055752 over the end of the valve 6 To reinstall the valve containing the new O ring turn the knob clockwise and then tighten fingertight NOTE It is normal to encounter resistan...

Page 135: ...cover The screws remain attached see Figure 5 16 View B Figure 5 16 Removing the Cell Front Cover 3 Grip the two loosened screws and pull the cell cover toward you to remove it from the component pane...

Page 136: ...e slope calibration which measures the output of the cell electronics when a built in dummy cell which provides a constant conductivity reading of 21 S is automatically enabled The slope value is appl...

Page 137: ...n is complete touch Run Slope Cal d Touch the Cell Constant field and enter the value printed on the front of the cell e To ensure that the new calibration values are recorded in the ICS 2000 memory d...

Page 138: ...of the ICS 2000 4 Disconnect the two eluent and the two regenerant lines from the suppressor 5 To remove the suppressor from the component mounting panel slide it up to detach it from the mounting tab...

Page 139: ...Some suppressors require more force to secure them onto the tabs 13 Connect the two eluent and two regenerant lines to the new suppressor 14 Close the ICS 2000 front door Figure 5 20 Suppressor Guide...

Page 140: ...component panel 4 Before installing the new column heater write down the two calibration values offset and slope recorded on the back of the heater 5 Align the connector on the back of the new column...

Page 141: ...ext to the Download button verify that Current is selected and then click Download to download the new calibration values to the ICS 2000 d To ensure the new calibration values are recorded in the ICS...

Page 142: ...bscrews on the column heater cover they remain attached to the cover 4 Pull the heater cover straight out to remove it 5 Unscrew the six thumbscrews on the top metal plate see Figure 5 23 and remove t...

Page 143: ...er to the ICS 2000 2 Open the ICS 2000 front door 3 To prevent an eluent leak during the valve replacement procedure disconnect the eluent line from the reservoir cap Or plug the line on the left side...

Page 144: ...screwing and removing the two screws Save the screws 9 Thread the cable from the new eluent valve through the mounting plate 10 Attach the mounting plate to the new valve using the screws removed in S...

Page 145: ...he cable attached to the leak sensor is connected to a matching cable inside the ICS 2000 Continue pulling the cable until the connectors for the two cables are outside of the panel see Figure 5 28 6...

Page 146: ...Click Pump On on the Chromeleon Control panel to turn on the pump motor 4 Open the priming valve on the primary pump head by turning it one quarter to one half turn counterclockwise 5 Use the syringe...

Page 147: ...squeeze the tab on the top of the holder to release it and pull the holder straight out of its compartment Isopropyl alcohol may damage some columns Be sure to thoroughly rinse the alcohol from the p...

Page 148: ...artridge To replace a KOH LiOH MSA or NaOH EluGen cartridge go to Section 5 17 1 To replace a K2CO3 cartridge go to Section 5 17 2 The EluGen cartridge contains one of the following a corrosive base K...

Page 149: ...EluGen cartridge VENT line see Figure 5 31 from the EluGen cartridge by unscrewing the luer lock from the luer adapter If necessary use a wrench to hold the luer adapter in place while unscrewing the...

Page 150: ...7 Turn the cartridge upside down and set it in the EluGen cartridge service area on top of the ICS 2000 see Figure 5 32 and Figure 5 33 Figure 5 32 EluGen Cartridge Service Area Figure 5 33 EluGen Ca...

Page 151: ...provided on the Dionex Reference Library CD ROM P N 053891 to prepare an expended EluGen cartridge for disposal or to store a partially used EluGen cartridge for later use 10 Remove the new EluGen car...

Page 152: ...ridge INLET port 14 Locate the yellow 0 5 mL min 7 MPa 1000 psi backpressure coil P N 053765 in the ICS 2000 Ship Kit P N 064375 15 Connect one end of this coil to the EluGen cartridge OUTLET port see...

Page 153: ...der see Figure 5 38 NOTE To avoid crimping the lines inside the holder do not twist the cartridge before you slide it into the holder The lines are designed to coil inside the holder Figure 5 38 Insta...

Page 154: ...l cable connector as shown in Figure 5 39 Push it onto the EGC 1 connector on the ICS 2000 Twist the ring on the cable connector fingertight to secure it Figure 5 39 Connecting the EluGen Cartridge Ca...

Page 155: ...Configuration program select Start Programs or All Programs Chromeleon Server Configuration 2 Double click the ICS 2000 system icon The properties dialog box for the ICS 2000 appears 3 Click the Elue...

Page 156: ...fy that the suppressor and CR TC are off 4 Verify that the Automatically turn on with pump options are not selected on the ICS 2000 touch screen SUPPRESSOR page see Section C 9 and ELUENT GENERATOR pa...

Page 157: ...t see Figure 5 41 Figure 5 41 EluGen Cartridge Inlet and Outlet Connections 14 Turn over the EluGen cartridge fittings facing down Check for bubbles If necessary shake and tap the EluGen cartridge to...

Page 158: ...Remove the EluGen cartridge VENT line see Figure 5 31 from the EluGen cartridge by unscrewing the luer lock from the luer adapter If necessary use a wrench to hold the luer adapter in place while uns...

Page 159: ...cartridge upside down and set it in the EluGen cartridge service area on top of the ICS 2000 see Figure 5 32 and Figure 5 33 Figure 5 43 EluGen Cartridge Service Area Figure 5 44 EluGen Cartridge In t...

Page 160: ...tridge manual provided on the Dionex Reference Library CD ROM P N 053891 to prepare an expended EluGen cartridge for disposal or to store a partially used EluGen cartridge for later use 10 Remove the...

Page 161: ...slodged Figure 5 47 EluGen Cartridge in Service Area The EluGen cartridge contains one of the following a corrosive base KOH LiOH or NaOH a corrosive acid MSA or a concentrated K2CO3 solution Wear pro...

Page 162: ...ned to coil inside the holder Figure 5 48 EluGen Cartridge Installed in the Holder 17 Remove the plug from the EGC 1 connector on top of the ICS 2000 18 Orient the cartridge s blue 4 pin electrical ca...

Page 163: ...3 Click the Eluent Generator tab 4 Select the EGC 1 check box 5 Enter the EluGen cartridge serial number Verify that the cartridge type is Carb If not make sure the cartridge serial number has been en...

Page 164: ...DEGAS EGC IN Connected to Gas Separator Assembly TO DEGAS ELUENT OUT TO SRS AES REGEN OUT WASTE GAS SEPARATOR Connected to Suppressor Regen Out Pre plumbed Pump Pulse Damper to Inlet Outlet EGC VENT...

Page 165: ...N 042627 in the ICS 2000 Ship Kit P N 064375 3 Use the coupler to connect the TO INJ VALVE IN P ELUENT OUT line to one end of the backpressure coil see Figure 5 51 4 Direct the free end of the backpr...

Page 166: ...ically turning on the suppressor 4 From the ICS 2000 Control panel in Chromeleon or the ICS 2000 touch screen HOME page set the pump flow rate to 1 00 mL min 5 Turn on the pump 6 From the Chromeleon C...

Page 167: ...EGC VENT TO DEGAS ELUENT IN EGC DEGAS Connected to Gas Separator Assembly Connected Inject Valve Port P 2 to Pre plumbed Pump Pulse Damper to Inlet Outlet Send to waste EGC CO Mixer 3 EGC II K CO 2 3...

Page 168: ...2 to 1 4 28 coupler to the free end of the backpressure coil 5 Disconnect the TO SRS AES REGEN OUT line from the suppressor REGEN OUT port 6 Connect the TO SRS AES REGEN OUT line to the 10 32 to 1 4 2...

Page 169: ...0 touch screen HOME page set the pump flow rate to 1 00 mL min 5 Turn on the pump 6 Select the following settings from the Chromeleon Control panel or the touch screen ELUENT GENERATOR page Set the EG...

Page 170: ...on the injection valve and disconnect the line labeled SRS AES REGEN OUT from the REGEN OUT port on the suppressor 4 Lift up the holder and tilt it to view the bottom of the holder 5 Brace the holder...

Page 171: ...stalled in Holder 11 Feed the CR TC cable through the slot on the side of the EGC holder see Figure 5 56 12 Turn the EGC holder right side up Figure 5 56 CR TC Cable 13 Reconnect the EGC IN and EGC OU...

Page 172: ...ed Type 1 18 megohm cm deionized water through the CR TC for 10 minutes while bypassing the columns and suppressor Figure 5 57 illustrates the liquid flow path through the CR TC during the hydration p...

Page 173: ...urning on the CR TC 5 Turn on the pump and set the flow to the rate recommended for your application Pump deionized water through the CR TC for at least 10 minutes 6 Turn off the pump and disconnect t...

Page 174: ...ing bracket with the EPM attached and remove the bracket and EPM from the EGC holder 5 Pull the EPM off the mounting bracket 6 Remove the plugs from the ports on the new EPM P N 063175 7 Push the EPM...

Page 175: ...ms Chromeleon Server Configuration 2 Double click the ICS 2000 system icon The properties dialog box for the ICS 2000 appears 3 Click the Eluent Generator tab see Figure 5 40 4 Select the EGC 2 check...

Page 176: ...DEGAS Connected to Gas Separator Assembly Connected Inject Valve Port P 2 to Pre plumbed Pump Pulse Damper to Inlet Outlet Send to waste EGC CO Mixer 3 EGC II K CO 2 3 TO CR TC EPM REGEN IN orange lab...

Page 177: ...sure coil 4 Disconnect the TO SRS AES REGEN OUT line from the suppressor REGEN OUT port 5 Connect the TO SRS AES REGEN OUT line to the 10 32 to 1 4 28 coupler see Figure 5 61 NOTE The backpressure coi...

Page 178: ...ontrol panel in Chromeleon or the ICS 2000 touch screen HOME page set the pump flow rate to 1 00 mL min 6 Turn on the pump 7 Run the pump for 5 minutes to hydrate the EPM 8 Select the following settin...

Page 179: ...ontrol panel in Chromeleon or from the touch screen HOME page 2 Disconnect the inlet and outlet lines labeled TO EGC CO3 MIXER IN and TO EGC CO3 MIXER OUT from the existing mixer and pull the mixer of...

Page 180: ...emporarily fill the EGC CO3 Mixer with deionized water Then follow the steps in the next section to fill the mixer with eluent of the required concentration for the application to be run 1 Verify that...

Page 181: ...7 03 4 06 5 71 3 Disconnect the TO EGC CO3 MIXER IN line from the TO INJ VALVE IN P ELUENT OUT line Figure 5 63 Disconnect the Cartridge Inlet and Pump Outlet TO PUMP DAMPER disconnect this line Pump...

Page 182: ...t the suppressor is off 9 Verify that the Automatically turn on with pump option is not selected on the ICS 2000 touch screen SUPPRESSOR page see Section C 9 Disabling this option lets you turn on the...

Page 183: ...initial use follow the steps in this section to fill the EGC CO3 Mixer with the eluent required for the application to be run The procedure varies slightly depending on the type of eluent to be gener...

Page 184: ...al Filling of the EGC CO3 Mixer with Carbonate Eluent EGC DEGAS EGC IN Connect to Gas Separator Assembly TO DEGAS ELUENT OUT TO SRS AES REGEN OUT WASTE GAS SEPARATOR Connect to Suppressor Regen Out Pr...

Page 185: ...n is not selected on the ICS 2000 touch screen SUPPRESSOR page see Section C 9 Disabling this option lets you turn on the pump without automatically turning on the suppressor 7 From the ICS 2000 Contr...

Page 186: ...2 mm mixer NOTE The void volume of the 4 mm mixer is about 16 mL The void volume of the 2 mm mixer is about 5 mL 13 Turn off the EGC 1 current 14 Turn off the pump 15 Remove the backpressure coil from...

Page 187: ...AS ELUENT OUT WASTE GAS SEPARATOR TO INJ VALVE IN P ELUENT OUT TO DEGAS ELUENT IN EGC DEGAS Connect to Gas Separator Assembly Pre plumbed Pump Pulse Damper to EGC CO Mixer 3 TO CR TC EPM REGEN IN oran...

Page 188: ...pressure coil to the TO INJ VALVE IN P ELUENT OUT line see Figure 5 67 3 Connect the other end of the backpressure coil to the coupler on the mixer inlet 4 Use the 10 32 to 1 4 28 coupler to connect t...

Page 189: ...0 mM 11 Turn on the EGC 1 and EGC 2 currents 12 Run at the selected settings until the mixer is filled with the eluent mixture At a flow rate of 1 mL min it will take about 45 minutes to fill the 4 m...

Page 190: ...isconnect the line labeled WASTE GAS SEPARATOR from the gas separator waste tube assembly installed on the waste container 3 Disconnect the EluGen cartridge cable and the CR TC or EPM cable if install...

Page 191: ...053981 provided with the EluGen cartridge onto the luer adapter This prevents leaks from the vent opening when you turn over the EluGen cartridge see Step 8 6 If an EPM is installed lift up the brack...

Page 192: ...ift the EGC holder out of the top cover and pull the liquid lines through the tubing chase see Figure 5 70 to remove them from the ICS 2000 Figure 5 70 Top Cover Tubing Chase 11 If a CR TC is installe...

Page 193: ...id lines from the four ports on the CR TC see Figure 5 54 Figure 5 71 Disconnecting CR TC Liquid Lines 4 Turn the new holder upside down see Figure 5 72 and connect the liquid lines to the CR TC NOTE...

Page 194: ...gure 5 73 CR TC Installed in Holder 6 Feed the CR TC cable through the slot on the side of the EGC holder see Figure 5 74 Figure 5 74 CR TC Cable 7 Turn the EGC holder right side up and set it on top...

Page 195: ...21 5 5 21 3 Removing the EPM and Reinstalling it in the New EGC Holder 1 Turn the EGC holder upside down to view the connections on the bottom of the holder 2 Disconnect the following lines that conne...

Page 196: ...er from each line just before connecting the line to a port Figure 5 76 EGC Holder upside down 6 Connect the lines from the EPM red yellow and blue labels to the corresponding lines in the holder 7 Di...

Page 197: ...the line labeled TO PUMP DAMPER to the pulse damper 12 Direct the WASTE GAS SEPARATOR line from the top of the ICS 2000 to the rear panel Snap the line onto one of the tubing clips on the rear panel...

Page 198: ...er Disconnect the EGC IN line from the coupler that connects it to the EGC OUT line 2 Connect the EGC IN line to the EluGen cartridge INLET port see Figure 5 77 3 Remove the coupler and connect the EG...

Page 199: ...justment Fuses Two 3 15 amp fast blow IEC 127 fuses P N 954745 Dimensions without reservoir or EluGen cartridge Height 56 1 cm 22 1 in Width 22 4 cm 8 8 in Depth 53 3 cm 21 0 in Weight 30 kg 66 lb Dec...

Page 200: ...pe Dual piston in series variable speed 100 L per revolution Flow Rate Settable range 0 00 to 5 00 mL min without eluent generator 0 10 to 3 00 mL min with eluent generator in 0 01 increments Optimal...

Page 201: ...ion 0 999 RSD 5 to 800 S Cell Body PEEK Electrodes Passivated 316 stainless steel Active Volume 1 0 L Operating Temperature 30 to 55 C 86 to 130 F must be at least 7 C above the ambient temperature Ma...

Page 202: ...1 to 15 mM at 0 1 to 1 0 mL min flow 0 1 to X mM at 1 0 to 2 0 mL min flow where X 15 flow in mL min KOH 0 1 to 100 mM at 0 1 to 1 0 mL min flow 0 1 to X mM at 1 0 to 3 0 mL min flow where X 100 flow...

Page 203: ...d the specified range The EPM concentration must not exceed 10 mM KOH KOH KOH MSA KOH NaOH MSA MSA MSA NaOH NaOH NaOH 0 1 to 50 mM at 0 1 to 1 0 mL min flow 0 1 to X mM at 1 0 to 3 0 mL min flow X 50...

Page 204: ...ICS 2000 Ion Chromatography System A 6 Doc 031857 03 4 06...

Page 205: ...sure the ICS 2000 installation site meets the power and environmental specifications listed in Appendix A Provide a sturdy workbench for the ICS 2000 of a height that ensures convenient access to the...

Page 206: ...g the top layer of foam remove the next layer of foam see Figure B 1 Figure B 1 Unpacking the ICS 2000 2 Set the eluent bottle on top of the ICS 2000 see Figure B 2 3 TURN OVER THE EGC HOLDER and set...

Page 207: ...he ICS 2000 which weighs 30 kg 66 lb Lift the ICS 2000 only from each side of the cabinet bottom Lifting from the front door will damage the door hinges Au moins deux personnes peuvent soulever le ICS...

Page 208: ...icense B 2 2 Unpacking the Computer outside North America 1 Refer to Installing the Chromeleon IC System Document No 031883 to verify that the computer being used meets Chromeleon specifications The m...

Page 209: ...pear on the screen 3 Verify that the monitor display is set to a minimum of 1024 x 768 with 16 bit color 4 Insert the Chromeleon CD ROM into the CD ROM drive 5 The Chromeleon Setup menu appears 6 If t...

Page 210: ...screws on a parallel port dongle 2 Microsoft Windows will automatically detect the USB dongle and launch the Found New Hardware Wizard Complete the wizard by selecting the following options If asked...

Page 211: ...sary to insert a hardware installation CD or floppy disk Figure B 4 Windows Found New Hardware Wizard 3 If it is not already running start the Chromeleon Server Monitor by double clicking the Server M...

Page 212: ...was entered before shipping the computer and you can omit the remaining steps in this section Click Close and go on to Section B 5 If Chromeleon Server is running idle Evaluation Mode is displayed see...

Page 213: ...tab and click Dongle Enter the Key Code exactly as it appears on the Chromeleon Software License key code sheet Click OK 8 Check the Server Monitor status It should now display Chromeleon Server is ru...

Page 214: ...lug the cable s B connector into the USB receptacle labeled FROM PC on the ICS 2000 rear panel see Figure B 9 Do not turn on the power to the ICS 2000 or other USB module s until after you connect the...

Page 215: ...e number of USB devices in the system exceeds the number of available USB ports connect the devices to the system with a USB compliant full speed external hub One or more hubs is also required if the...

Page 216: ...sible Operation at AC input levels outside of the specified operating voltage range may damage the ICS 2000 DANGER D LECTROCUTION Pour viter toute lectrocution il faut utiliser une prise de courant av...

Page 217: ...ing Start Server When the server starts the icon changes to gray 3 Press the power switch on the ICS 2000 rear panel see Figure B 11 to turn on the power Figure B 11 ICS 2000 Rear Panel 4 Windows auto...

Page 218: ...electing the following options If asked whether Windows can connect to Windows Update to search for software select No not this time Select the Install the software automatically Recommended option se...

Page 219: ...ese steps refer to the Chromeleon user s guide or online Help B 8 1 Assigning the ICS 2000 to a Timebase After the ICS 2000 power is turned on and the Windows Found New Hardware Wizard is finished the...

Page 220: ...uration Wizard Page 2 2 Review the names displayed in the Unassigned USB Devices Detected window If the list includes the name of a device that you do not want to configure now clear the check mark be...

Page 221: ...guration program is already running 5 Click Accept to create a new timebase The Server Configuration automatically starts running when you close the wizard If the Server Configuration was already runn...

Page 222: ...name to expand the folder The timebase you created is displayed see Figure B 15 Figure B 15 Server Configuration with ICS 2000 Timebase 2 To verify that the ICS 2000 is correctly connected to the tim...

Page 223: ...ump_ECD the Mode Live and the Module Serial No 4 Close the dialog box and then save the configuration select File Save Installation 5 If you are going to add one or more DX LAN devices to the timebase...

Page 224: ...through USB connections 1 Follow the instructions in Installing the Chromeleon Chromatography Management System with a Dionex Ion Chromatography IC Document No 031883 to add the DX LAN card to the se...

Page 225: ...odules and then select the device from the Devices list 4 Click OK A configuration properties dialog box for the device appears Figure B 19 shows an example of the properties for an AS50 Autosampler F...

Page 226: ...eck box if it is currently selected and then select the DX LAN Device ID number Note Devices display their DX LAN ID numbers when their power is turned on 6 Click OK to close the dialog box 7 Save the...

Page 227: ...eference Library CD ROM P N 053891 Before beginning Remove the guard column separator column and suppressor from their boxes Remove the fitting plugs from the ends of each column and from all ports on...

Page 228: ...lumn inlet Check the arrow on the column label it should point down flow away from the injection valve 3 Connect GUARD OUT to the guard column outlet 4 Push the guard column onto the back column clip...

Page 229: ...pressor align the pins on the two connectors and push them together Figure B 22 Suppressor Mounting Tabs and Guidelines 3 Orient the suppressor with the REGEN OUT fitting on top and the cable to the r...

Page 230: ...he coupler connecting ELUENT IN and ELUENT OUT and connect these lines to the corresponding ports on the suppressor Figure B 23 Suppressor Installed SRS Shown 8 Remove the fitting plug from REGEN IN a...

Page 231: ...o prevent waste from siphoning back into the system check the lines periodically to be sure they are not bent pinched or elevated at any point B 10 1 Installing the Gas Separator Waste Tube 1 Locate t...

Page 232: ...nerating Suppressor SRS travaillent avec un proces d lectrolyse qui produit des petites quantit s de gaz d oxyg ne ou d hydrog ne Afin de garantir que le gaz ne soit pas enferm dans un conteneur ferm...

Page 233: ...e I 18 megohm deionized water 3 Place the reservoir on top of the ICS 2000 4 Install the cap and handtighten NOTE The ICS 2000 does not require pressurized reservoirs However if eluent is manually deg...

Page 234: ...rning is displayed if the level falls below 200 mL The warning is repeated at 100 mL and at 0 mL Go on to Section B 13 In order for the eluent level displayed on the screen to be accurate the level mu...

Page 235: ...connected see Section B 11 2 Verify that the waste lines are directed to a waste container 3 The priming procedure consists of two parts Priming the eluent lines with a syringe see Section B 13 1 Per...

Page 236: ...2 Connect a 10 mL syringe P N 054578 to the port in the priming valve on the primary pump head see Figure B 27 3 Open the priming valve by turning it one quarter to one half turn counterclockwise 4 On...

Page 237: ...through the flow path It may take several syringe draws to remove all air or previous eluent from the tubing Draw out a minimum of 20 mL of eluent to ensure that the vacuum degas assembly is primed 7...

Page 238: ...ates backpressure 3 Touch Prime on the ICS 2000 touch screen HOME page Confirm that the waste valve is open by pressing OK when the reminder message appears The pump will begin pumping at approximatel...

Page 239: ...ng conditions for your application The manuals are provided on the Dionex Reference Library CD ROM P N 053891 The EluGen cartridge contains one of the following a corrosive base KOH LiOH or NaOH a cor...

Page 240: ...bonate bicarbonate eluent go to Section B 14 2 If you are installing a K2CO3 cartridge and an EGC CO3 Mixer for generating carbonate eluent go to Section B 14 3 Overview The eluent generator setup pro...

Page 241: ...it P N 064375 053765 Provide backpressure during setup procedures EluGen Cartridge Vent Line Tubing Kit supplied with the cartridge 063092 Vent the cartridge during operation 10 32 to 1 4 28 coupler s...

Page 242: ...8 Doc 031857 03 4 06 Figure B 30 illustrates the ICS 2000 after the EluGen cartridge is installed Figure B 30 ICS 2000 with an EluGen Cartridge Installed Top View IN EGC OUT EluGen Cartridge EGC EGC H...

Page 243: ...EGC IN Connect to Gas Separator Assembly TO DEGAS ELUENT OUT TO CR TC REGEN OUT blue TO SRS AES REGEN OUT TO CR TC REGEN IN orange WASTE GAS SEPARATOR DEGAS ELUENT OUT TO INJ VALVE IN P ELUENT OUT Con...

Page 244: ...etal ball stud inside the bottom of the cartridge holder see Figure B 32 and the bracket on the CR TC mounting plate see Figure B 33 These are used to mount the CR TC inside the holder after it is plu...

Page 245: ...ngs and ferrules on the lines remove the coupler from each line just before connecting the line to a port Figure B 34 Plumbed CR TC 5 Check that none of the lines are caught under the CR TC and then p...

Page 246: ...urn the EGC holder right side up and set it back into position Installing the EluGen Cartridge 1 Orient the EluGen cartridge with the fittings facing up and remove the plugs from the EluGen cartridge...

Page 247: ...e EGC holder see Figure B 38 3 Pull the coiled black EGC IN and EGC OUT lines out slightly from the cartridge holder 4 Disconnect the EGC IN line from the union that connects it to the EGC OUT line 5...

Page 248: ...tridge vigorously and tap it with the palm of your hand 10 to 15 times Check that all bubbles trapped in the eluent generation chamber are dislodged The EluGen cartridge contains one of the following...

Page 249: ...e cartridge when you slide it into the holder The lines are designed to coil inside the holder Figure B 41 Installing the EluGen Cartridge in the Holder 10 Remove the plug from the EGC 1 connector on...

Page 250: ...opening Remove the cap on the luer adapter by twisting Save the cap 4 Twist the luer lock and EluGen cartridge vent line into the luer adapter see Figure B 44 Figure B 44 Installing the EluGen Cartrid...

Page 251: ...leon Server Configuration program select Start Programs or All Programs Chromeleon Server Configuration 2 Double click the ICS 2000 system icon The properties dialog box for the ICS 2000 appears 3 Cli...

Page 252: ...h screen SUPPRESSOR page see Section C 9 and ELUENT GENERATOR page see Section C 8 Disabling these options lets you turn on the pump without automatically turning on the suppressor and CR TC 6 Verify...

Page 253: ...ssary shake and tap the EluGen cartridge to remove bubbles 18 Reinstall the EluGen cartridge in the holder 19 Do not reconnect the EluGen cartridge cable until after hydrating the CR TC Hydrating the...

Page 254: ...R page see Section C 9 Disabling this option lets you turn on the pump without automatically turning on the suppressor Figure B 47 CR TC Plumbing for Hydration To avoid damaging the suppressor always...

Page 255: ...econnect the EluGen cartridge cable 4 Remove the plug from the CR TC connector on top of the ICS 2000 5 Orient the CR TC s black 3 pin electrical cable connector as shown in Figure B 42 Push it onto t...

Page 256: ...H MSA or NaOH EluGen cartridge and a CR TC go to Section B 14 1 Overview The eluent generator setup procedure for carbonate bicarbonate eluent consists of the following main steps Installing the EluGe...

Page 257: ...e EPM REGEN OUT port EluGen Cartridge Vent Line Tubing Kit supplied with the cartridge 063092 Vent the cartridge during operation Yellow 0 5 mL min 7 MPa 1000 psi backpressure coil 053765 Provide back...

Page 258: ...romatography System B 54 Doc 031857 03 4 06 Figure B 49 illustrates the EluGen cartridge and EPM after installation Figure B 49 ICS 2000 with an EluGen Cartridge and EPM Installed EluGen Cartridge EGC...

Page 259: ...EGC VENT TO DEGAS ELUENT IN EGC DEGAS Connect to Gas Separator Assembly Connect Inject Valve Port P 2 to Connect to Suppressor Regen Out Pre plumbed Pump Pulse Damper to Inlet Outlet Send to waste EGC...

Page 260: ...B 56 Doc 031857 03 4 06 Installing the EluGen Cartridge 1 Tilt the cartridge holder to view the bottom and brace the holder against the back corner of the ICS 2000 see Figure B 51 Figure B 51 Cartridg...

Page 261: ...tubing red labels P N 064334 to the coupler see Figure B 63 b Connect the 35 cm 14 in length of black PEEK tubing yellow labels P N 064340 to the coupler on the TO CR TC ELUENT OUT line yellow label...

Page 262: ...of the holder see Figure B 53 5 Turn the EGC holder right side up and set it back into position 6 Orient the EluGen cartridge with the fittings facing up and remove the plugs from the cartridge INLET...

Page 263: ...GC IN and EGC OUT lines out from inside the EGC holder Disconnect the EGC IN line from the coupler that connects it to the EGC OUT line 9 Connect the EGC IN line to the EluGen cartridge INLET port see...

Page 264: ...s are designed to coil inside the holder Figure B 57 EluGen Cartridge Installed in the Holder The EluGen cartridge contains one of the following a corrosive base KOH LiOH or NaOH a corrosive acid MSA...

Page 265: ...the cable connector fingertight to secure it Figure B 58 Connecting the EluGen Cartridge Cable Connecting the EluGen Cartridge Vent Line 1 Locate the EluGen Cartridge Vent Line Tubing Kit supplied wit...

Page 266: ...E To prevent leaks from the EluGen cartridge always replace the luer adapter cap before moving the EluGen cartridge 6 Direct the vent line to the waste container and insert it down into the gas separa...

Page 267: ...talled on EGC Holder 4 Connect the following lines to the EPM Connect the TO CR TC EPM ELUENT OUT line yellow label to the ELUENT OUT port on the EPM see Figure B 63 Connect the TO CR TC EPM ELUENT IN...

Page 268: ...Gas Separator Assembly Pre plumbed Pump Pulse Damper to Inlet Outlet Send to waste EGC II K CO 2 3 TO CR TC EPM REGEN IN orange label TO PUMP DAMPER TO CR TC EPM ELUENT OUT yellow label TO CR TC EPM...

Page 269: ...ogram select Start Programs or All Programs Chromeleon Server Configuration 2 Double click the ICS 2000 system icon The properties dialog box for the ICS 2000 appears 3 Click the Eluent Generator tab...

Page 270: ...TO DEGAS ELUENT OUT WASTE GAS SEPARATOR TO INJ VALVE IN P ELUENT OUT EGC VENT TO DEGAS ELUENT IN EGC DEGAS Connect to Gas Separator Assembly Pre plumbed Pump Pulse Damper to Inlet Outlet Send to wast...

Page 271: ...re coil 4 Disconnect the TO SRS AES REGEN OUT line from the suppressor REGEN OUT port 5 Connect the TO SRS AES REGEN OUT line to the 10 32 to 1 4 28 coupler see Figure B 66 NOTE The backpressure coil...

Page 272: ...e set the pump flow rate to 1 00 mL min 5 Turn on the pump 6 Run the pump for 5 minutes to hydrate the EPM 7 Touch the EGC button on the HOME page to open the ELUENT GENERATOR page 8 Verify that the c...

Page 273: ...rn off the EGC 1 and EGC 2 current 13 Turn off the pump Installing the EGC CO3 Mixer Figure B 67 illustrates the EGC CO3 Mixer after installation Figure B 67 ICS 2000 with an EGC CO3 Mixer Installed T...

Page 274: ...the outlet pointing upward and push it onto the clips see Figure B 69 The mixer outlet must point upward to ensure thorough mixing of the eluent 4 Locate the 35 cm 14 in length of black PEEK tubing w...

Page 275: ...rify that the EGC 1 and EGC 2 currents are off 2 Disconnect the EluGen cartridge inlet line labeled TO PUMP DAMPER from the coupler that connects it to the green pump outlet line see Figure B 70 Figur...

Page 276: ...ou turn on the pump without automatically turning on the suppressor 7 Set the pump flow rate to 5 00 mL min 8 Turn on the pump 9 Run the pump until the mixer is filled and there is a consistent flow o...

Page 277: ...2 EluGen Cartridge and EPM Plumbing for Initial Filling of the EGC CO3 Mixer with Carbonate Bicarbonate Eluent EGC IN TO DEGAS ELUENT OUT WASTE GAS SEPARATOR TO INJ VALVE IN P ELUENT OUT EGC VENT TO D...

Page 278: ...m the TO SRS AES REGEN OUT line and then connect the backpressure coil to the mixer inlet see Figure B 73 5 Connect the mixer outlet to the TO SRS AES REGEN OUT line NOTE The backpressure coil connect...

Page 279: ...mM and the EGC 2 concentration to 1 0 mM 12 Turn on the EGC 1 and EGC 2 currents 13 Run at the selected settings until the mixer is filled with the eluent mixture At a flow rate of 1 mL min it will ta...

Page 280: ...0 Flow Schematic Carbonate Bicarbonate Eluent Generation Eluent In Regen In Regen Out Eluent Out L L S W P C EPM Eluent In Eluent Out Regen Out Regen In To the pump Degas In Cell In Cell Out Column He...

Page 281: ...t line to the back of the component panel see Figure B 75 3 Connect the mixer outlet line to port P 2 on the injection valve NOTE Backpressure tubing may be required between the injection valve and th...

Page 282: ...R TC go to Section B 14 1 If you are installing a K2CO3 cartridge an EPM and an EGC CO3 Mixer for generating carbonate bicarbonate eluent go to Section B 14 2 Overview The eluent generator setup proce...

Page 283: ...ng operation EGC CO3 Mixer mounting clips 063642 Install the mixer Phillips head screwdriver not supplied n a Install the EGC CO3 Mixer mounting clips 35 cm 14 in of black 0 25 mm 0 010 in ID PEEK tub...

Page 284: ...DEGAS EGC IN Connect to Gas Separator Assembly TO SRS AES REGEN OUT WASTE GAS SEPARATOR Pre plumbed Pump Pulse Damper to Inlet Outlet EGC VENT Send to waste EGC OUT EGC II K CO 2 3 TO DEGAS ELUENT IN...

Page 285: ...path during the conditioning procedure Figure B 77 EluGen Cartridge Plumbing for Conditioning with Carbonate Eluent EGC DEGAS EGC IN Connect to Gas Separator Assembly TO DEGAS ELUENT OUT TO SRS AES R...

Page 286: ...move the plugs from the cartridge INLET and OUTLET fittings see Figure B 54 Figure B 78 EluGen Cartridge Fitting Plugs 2 Set the EluGen cartridge in the service area on top of the ICS 2000 and orient...

Page 287: ...the palm of your hand 10 to 15 times Check that all bubbles trapped in the eluent generation chamber are dislodged The EluGen cartridge contains one of the following a corrosive base KOH LiOH or NaOH...

Page 288: ...en you slide it into the holder The lines are designed to coil inside the holder Figure B 81 EluGen Cartridge Installed in the Holder 8 Remove the plug from the EGC 1 connector on top of the ICS 2000...

Page 289: ...nect the TO INJ VALVE IN P ELUENT OUT line to one end of the yellow 0 5 mL min 7 MPa 1000 psi backpressure coil P N 053765 see Figure B 83 12 Direct the free end of the backpressure coil to a temporar...

Page 290: ...84 Figure B 84 EluGen Cartridge Vent Plug 4 Screw the luer adapter onto the vent opening Remove the cap on the luer adapter by twisting Save the cap 5 Twist the luer lock and EluGen cartridge vent li...

Page 291: ...umber is printed on the EluGen cartridge label 1 Open the Chromeleon Server Configuration program select Start Programs or All Programs Chromeleon Server Configuration 2 Double click the ICS 2000 syst...

Page 292: ...n the ICS 2000 touch screen HOME page set the pump flow rate to 1 00 mL min 5 Turn on the pump 6 On the touch screen HOME page touch the EGC button to open the ELUENT GENERATOR page Verify that the ca...

Page 293: ...Doc 031857 03 4 06 B 89 Installing the EGC CO3 Mixer Figure B 67 illustrates the EGC CO3 Mixer after installation Figure B 87 ICS 2000 with an EGC CO3 Mixer Installed TO EGC CO3 MIXER IN P N 064346 T...

Page 294: ...with the outlet pointing upward and push it onto the clips see Figure B 89 The mixer outlet must point upward to ensure thorough mixing of the eluent 4 Locate the 35 cm 14 in length of black PEEK tubi...

Page 295: ...nitial filling of the EGC CO3 Mixer with carbonate eluent Figure B 90 Plumbing for Filling the EGC CO3 Mixer with Carbonate Eluent EGC DEGAS EGC IN Connect to Gas Separator Assembly TO DEGAS ELUENT OU...

Page 296: ...If necessary adjust the pressure by adding or removing backpressure tubing 8 Set the EGC 1 concentration to the value required for your application 9 Turn on the EGC 1 current 10 Run at these setting...

Page 297: ...n Figure B 91 ICS 2000 Flow Schematic Carbonate Eluent Generation L L S W P C To the pump Degas In Eluent In Regen In Regen Out Eluent Out Cell In Cell Out Column Heater Suppressor Heat Exchanger Guar...

Page 298: ...let line to the back of the component panel see Figure B 92 3 Connect the mixer outlet line to port P 2 on the injection valve NOTE Backpressure tubing may be required between the injection valve and...

Page 299: ...ed to know the type of column and suppressor you will be using This section shows how to create a sequence a list of samples to be processed and how to use an application template 1 Identify the type...

Page 300: ...B 96 Doc 031857 03 4 06 3 In the Browser select File New and select Sequence using Wizard see Figure B 94 The Sequence Wizard Welcome page appears see Figure Figure B 94 Creating a New Sequence Figure...

Page 301: ...7 NOTE For details about a Sequence Wizard step click the Help button on the Sequence Wizard page 4 Click Next The Sequence Wizard Timebase page appears see Figure B 96 Figure B 96 Example Sequence Wi...

Page 302: ...Template for Sample Name field type Sample click the right arrow and select Sample Number n 8 In the Number of Vials field type 1 9 Use default settings for the remaining parameters on the page NOTE Y...

Page 303: ...p a Chromeleon Application Doc 031857 03 4 06 B 99 10 Click Apply Figure B 98 shows Sequence Wizard Step 2 after a sample is defined Figure B 98 Example Sequence Wizard Step 2 with Sample Defined 11 C...

Page 304: ...The Standard Samples page appears see Figure B 99 Figure B 99 Example Sequence Wizard Step 3 12 Click Next to omit this step and define standard injections later Or define one or more standard inject...

Page 305: ...or to be installed and then select the column In the example in Figure B 100 the 4 mm ASRS suppressor and AS17 column are selected Figure B 100 Example Sequence Wizard Step 4 15 To select a report tem...

Page 306: ...stem B 102 Doc 031857 03 4 06 17 Enter a name for the sequence and a location see the example in Figure B 101 Figure B 101 Example Sequence Wizard Step 5 18 Click Finish and then click Done on the las...

Page 307: ...ple data The qnt file quantification method contains peak identification and area determination parameters The pan file Control panel lets you control and or monitor the ICS 2000 and other modules tha...

Page 308: ...l Panel Example NOTE If the Connected check box is not selected click the box to connect the ICS 2000 to the Control panel B 15 1 Verifying Chromeleon Communication Verify that Chromeleon is communica...

Page 309: ...backpressure of 16 MPa 2300 psi is ideal If the pressure is too low install a backpressure coil see Section B 16 1 4 Check that liquid is flowing out of the suppressor REGEN OUT waste line and that t...

Page 310: ...to port P 2 on the injection valve connect the other end to the TO INJ VALVE IN P ELUENT OUT line or to the mixer outlet if an EGC CO3 Mixer is installed B 17 Verifying Operational Status After the s...

Page 311: ...of injection full loop partial loop partial loop limited sample and others are supported NOTE Dionex recommends using AS or AS50 USB models that do not have injection valves see the list below If a va...

Page 312: ...at extends from the inject port in the autosampler is coiled inside the autosampler door on the right side Route this line through the autosampler right side slot see Figure B 104 and the ICS 2000 lef...

Page 313: ...artment You can either leave the disconnected Teflon waste line and coupler in the tubing chase or pull the line out from the front Figure B 106 Injection Valve Waste Line Coupler 6 Push the end of th...

Page 314: ...pen the Chromeleon Server Configuration program and select the ICS 2000 device in the timebase b Select Properties on the Edit menu c Select the Inject Valve tab d Verify that the Pump_InjectValve che...

Page 315: ...tor plug from the pair of wires labeled LOAD on the relay control cable 6 Locate the 12 position connector plug P N 923686 in the ICS 2000 Ship Kit P N 064375 see Figure B 109 7 Attach the red wire fr...

Page 316: ...Connect the relay control cable s 10 position connector plug to the RELAY CONTROL connector on the AS40 rear panel see Figure B 110 9 Plug the 12 position connector plug into the TTL and relay connect...

Page 317: ...ation for the device for connection instructions Analog output is 0 to 1V scaled to the selected range see Section C 10 3 For an example setup for ICS 2000 stand alone operation with an AS or AS50 USB...

Page 318: ...to a clean gas source regulated to between 0 55 and 0 83 MPa 80 and 120 psi 3 Push the other end of the tubing onto the inlet of the air regulator see Figure B 112 4 Remove the eluent reservoir cap 5...

Page 319: ...the reservoir when the gas source is turned on 2 Turn on the gas source 3 Pull out the air regulator knob and turn it clockwise to pressurize the eluent reservoir 4 Adjust the pressure to between 0 0...

Page 320: ...h the pump heads 3 The flush solution is carried to the piston seal wash assembly and out to waste via 1 5 mm 0 06 in ID tubing P N 055847 Locate this tubing in the ICS 2000 Ship Kit P N 064375 and cu...

Page 321: ...seal and draw out all of the air e Push approximately 5 cm 2 in of black 0 25 mm 0 01 in ID tubing P N 057057 into the end of the waste line NOTE The black tubing creates a flow restriction Assuming...

Page 322: ...em configurations are provided 2 Open the panel for your system configuration by double clicking its name in the list For example if your system includes an ICS 2000 and an AS50 autosampler double cli...

Page 323: ...the name of the ICS 2000 timebase at the bottom of the menu The Control panel is now connected to the ICS 2000 and the controls are enabled see Figure B 116 Figure B 116 ICS 2000 Traditional System Co...

Page 324: ...ible Cause The USB cable was connected and the power turned on before Chromeleon was installed Solutions Follow these steps to resolve the problem 1 Click Cancel in the Windows message box 2 Turn off...

Page 325: ...ed by the INFORMATION page which displays version and serial numbers and a list of installed options After a few seconds the HOME page see Figure C 1 is displayed The HOME page provides controls for a...

Page 326: ...lly touch a button or field you can cancel the action by sliding your finger away from the button or field before lifting Touching a command button for example executes the command immediately Selecti...

Page 327: ...C 3 ICS 2000 Touch Screen Menu of Pages Touching a page name on the menu of pages displays the selected page For example touching SUPPRESSOR displays the SUPPRESSOR page see Figure C 4 Figure C 4 Supp...

Page 328: ...s from conflicting with Chromeleon commands In general when the ICS 2000 is connected to Chromeleon you can View all ICS 2000 operational status information pressure flow rate conductivity readings et...

Page 329: ...of ICS 2000 Touch Screen Pages FLOW RATE MENU Calibration Pages Diagnostic Pages DIAGNOSTIC PUMP DETECTOR HOME SUPPRESSOR EGC STATUS PLOT INFORMATION INPUT OUTPUT MODULE SETUP These pages can be open...

Page 330: ...rent pressure reading If the pressure reading goes outside these limits the pump is stopped and an error message appears in the Chromeleon Audit Trail and on the touch screen See Section 4 1 for troub...

Page 331: ...ming touch the Off button During priming the flow rate is approximately 3 mL min See Section B 13 for detailed priming instructions Eluent Level Use this field to monitor the amount of liquid in the e...

Page 332: ...adient delivery in which the eluent concentration changes over time is also possible To set the eluent concentration or to turn on or off the CR TC touch the concentration button The ELUENT GENERATOR...

Page 333: ...tivity reading is the background conductivity the eluent conductivity before sample injection Touch Autozero to zero the conductivity and offset this background reading from the total reading NOTE You...

Page 334: ...touch the suppressor button The SUPPRESSOR page appears see Section C 9 C 4 6 Other Home Page Controls This field displays Remote Mode when the ICS 2000 is connected to Chromeleon and Local Mode when...

Page 335: ...conductivity reading and sets the plot baseline to zero Subsequent output is plotted in relation to the new zero point Offsetting the plot does not affect the data sent to Chromeleon the analog output...

Page 336: ...reservoir installed After filling the reservoir go to the HOME page and enter the amount of liquid in the reservoir see Section C 4 1 The ICS 2000 monitors the eluent usage based on the flow rate and...

Page 337: ...arameters to display here Figure C 9 shows the default parameters Eluent time Flow rate EGC conc and Suppressor To select different parameters touch the Setup button See Section C 6 1 for details abou...

Page 338: ...tus Parameters Follow the steps below to view the status of a parameter that is not currently displayed on the STATUS page 1 Touch the Setup button A drop down list replaces the label for each field a...

Page 339: ...ill last if the pump runs continuously at the current flow rate Flow rate The actual flow rate currently output by the pump EGC1 conc EGC2 conc The actual concentration of eluent currently being gener...

Page 340: ...system pressure limit is 34 5 MPa 5000 psi and the minimum pressure limit is 0 The eluent generator however requires a maximum high limit of 20 7 MPa 3000 psi and a minimum low limit of 1 4 200 psi Wh...

Page 341: ...s By default the ICS 2000 monitors the degas pressure reading and turns the degas pump on and off as required To change the degas operating option 1 Select PUMP from the menu of pages 2 Under Degas Se...

Page 342: ...he ICS 2000 monitors the degas pressure reading and turns the degas pump on and off as required C 7 3 Controlling the Eluent Valve The eluent valve controls flow from the eluent reservoir During routi...

Page 343: ...Number The Serial Number field displays the serial number of the EluGen cartridge currently installed After installing a new EluGen cartridge enter the cartridge serial number If you use Chromeleon t...

Page 344: ...ration of eluent currently being generated The suppressor type EGC type and flow rate determine the maximum eluent concentration for a particular application To determine the eluent concentration for...

Page 345: ...of manufacture Although you can continue operation with the cartridge after the expiration date performance may be impaired until a new cartridge is installed Life Remaining displayed when an EluGen c...

Page 346: ...and On buttons on the ELUENT GENERATOR page see Figure C 17 To automatically control the CR TC power simultaneously with the pump flow select Auto turn on with pump NOTE The CR TC power always turns o...

Page 347: ...to be changed However if a different type of suppressor is installed later or if the suppressor is removed from the system touch the Type field and select the new suppressor type None MMS SRS or AES...

Page 348: ...ns To automatically control the suppressor power simultaneously with the pump flow select Automatically turn on with pump NOTE The suppressor always turns off when the pump is off regardless of whethe...

Page 349: ...e will contain much less short term noise However longer rise times may have the following effects on peaks Peak shape will become asymmetric The peak maximum will be shifted The peak height will be r...

Page 350: ...Zero to set the output signal to zero volts Select Full Scale to set the output signal to the full scale voltage 1000 mV Offset To adjust the zero position of the analog output when it is plotted ente...

Page 351: ...0 The INFORMATION page is displayed at start up You can open the page from the menu of pages at any time Moduleware is the firmware program installed in the ICS 2000 that controls instrument functions...

Page 352: ...reen Time out If the touch screen is not used during the selected time out the display will change to show the screen selected from the list For example if you select 5 min and BUSY the display will c...

Page 353: ...nput Output Page The INPUT OUTPUT page provides control of the ICS 2000 TTL inputs and TTL and relay outputs To open this page select INPUT OUTPUT from the menu of pages For details about relay and TT...

Page 354: ...r calibration page select DIAGNOSTIC from the menu of pages The DIAGNOSTIC page opens see Figure C 23 From there you can select the type of diagnostic or calibration to perform For detailed instructio...

Page 355: ...A or with included power supplies over 60 V may damage the relay drivers on the CPU 1 2 OUT RELAY TTL OUT 1 2 TTL GND 1 2 3 4 TTL IN 1 2 1 2 Description Solid State Relay Contacts Output Pin Function...

Page 356: ...TL or Relay 1 Locate the twisted pair of wires P N 043598 and the 12 position connector plug P N 923687 see Figure D 2 in the ICS 2000 Ship Kit P N 064375 2 Follow these basic steps to connect the TTL...

Page 357: ...rect function is assigned to the input and that the correct input control type is selected Select different settings if necessary Input functions and control types are assigned from either the INPUT O...

Page 358: ...the output signals provided by Dionex modules If the device connected to the ICS 2000 does not send a normal edge signal select the appropriate control type Refer to the documentation provided with th...

Page 359: ...guaranteed to be detected A pulse width of 4 ms or less is guaranteed to be ignored The action for pulse widths that are greater than 4 ms and less than 50 ms is undefined Inverted Pulse The inverted...

Page 360: ...0 autosampler Figure D 5 Example Program for AS40 Control To control the TTL and relay output states from the touch screen select INPUT OUTPUT from the menu of pages The INPUT OUTPUT page appears see...

Page 361: ...Ls and Relays 1 Locate two twisted pairs of wires P N 043598 and 12 position connector plugs P N 923686 see Figure D 6 in the ICS 2000 and AS or AS50 USB Ship Kits 2 Attach the wires to the connector...

Page 362: ...TTL Out 2 to ICS 2000 TTL In 2 AS50 RLY Out 2 to ICS 2000 TTL In 3 AS50 RLY Out 1 to Third party DAC RLY In Third party ADC RLY OUT Connection Analog Data Transfer AS50 Rear Panel Connections ICS 200...

Page 363: ...l Type is Normal Edge see Figure D 8 Enable AS or AS50 USB Control of the Injection Valve 1 On the AS or AS50 USB front panel press Menu and 0 to go to the TIME FUNCTION OUT screen In the LOAD INJECT...

Page 364: ...1 Locate the analog cable for the third party analog to digital converter and controller 2 Connect the analog input connector on the third party controller to the ANALOG OUTPUT connector on the ICS 2...

Page 365: ...not need to be set in the timed events because the Load and Inject commands control the ICS 2000 injection valve automatically see Enable AS or AS50 USB Control of the Injection Valve on page D 9 To...

Page 366: ...ICS 2000 Ion Chromatography System D 12 Doc 031857 03 4 06...

Page 367: ...Piston seal 048722 Piston rinse seal 055752 O ring for waste valve or priming valve 057945 Eluent valve 054578 10 cc syringe for priming eluent lines Eluent Generator 058904 EGC II K2CO3 EluGen Cartri...

Page 368: ...or 053949 CSRS Ultra 4 mm Cation Self Regenerating Suppressor 057985 DS6 heated conductivity cell 058060 Column heater assembly 059979 Column heater heat exchanger 0 25 mm 0 010 in ID tubing 060943 Co...

Page 369: ...on 4 6 for pump flow rate troubleshooting If a contaminated column is suspected clean the column as instructed in the column manual The column manual is included on the Dionex Reference Library CD ROM...

Page 370: ...rt on the Windows taskbar and then select Programs Chromeleon Chromeleon F 8 How do I delete data In the Chromeleon Browser highlight the sequence you want to delete and then select File Delete F 9 Ho...

Page 371: ...anual F 14 Why is the conductivity high Possible reasons for high conductivity include The suppressor needs regeneration See the suppressor manual for troubleshooting information The suppressor manual...

Page 372: ...ICS 2000 Ion Chromatography System F 4 Doc 031857 03 4 06...

Page 373: ...bing Calibration Curve A graph showing detector response in peak height or area versus analyte concentration Capacity Factor k The number of column volumes of eluent pumped through the column required...

Page 374: ...rable because resolution between closely spaced bands improves with greater efficiency For a symmetrical Gaussian peak column efficiency can be determined by the following N 5 54 t1 W1 2 2 Where t1 th...

Page 375: ...ductivity The contribution of an ionic species to the total conductivity of a solution as measured in a standard cell having electrodes 1 cm2 in area and exactly 1 cm apart Guard Column A small column...

Page 376: ...t conductivity Resin See Ion Exchange Resin Resolution R A measure of the separation between two sample components This is expressed as the ratio of the distance between the two peak maxima to the mea...

Page 377: ...fficient The percent of change in the conductivity of a solution with a 1 o C change in temperature Every solution has a characteristic temperature coefficient which is determined experimentally Theor...

Page 378: ...ICS 2000 Ion Chromatography System G 6 Doc 031857 03 4 06...

Page 379: ...Function Out screen D 9 Yearly maintenance 3 22 AS40 Automated Sampler Installation B 111 Sample injection 3 16 Yearly maintenance 3 22 Atlas Electrolytic Suppressor 2 9 See also Suppressor Audit Trai...

Page 380: ...cense installation B 5 B 6 Menu 2 12 3 18 Programs 2 12 2 15 3 16 3 18 Starting 3 4 B 95 System properties dialog box B 19 Timebase 3 5 Toolbars 2 12 3 18 Using the touch screen with Chromeleon C 4 Ve...

Page 381: ...C over current error 4 4 CR TC stopped for zero flow rate 4 4 Current C 23 D Daily maintenance 3 21 Data Backing up F 2 Deleting F 2 Filtering C 25 Saving 3 18 3 19 Data acquisition 3 19 Data analysis...

Page 382: ...4 EGC 2 connector 2 6 Elapsed time C 9 C 11 Electrical specifications Fuses A 1 Main power A 1 Electrolysis gases Purging from eluent 2 38 2 41 Electrolytic pH modifer See EPM Electrolytic pH Modifier...

Page 383: ...9 Pump over pressure 4 9 Pump pressure hardware error 4 9 Pump under pressure 4 10 Suppressor not connected 4 11 Suppressor over current 4 10 Suppressor over power 4 10 4 11 Suppressor stopped for zer...

Page 384: ...B 20 Analog output connection D 10 AS AS50 USB Autosampler B 107 AS40 autosampler B 111 Assigning devices to a timebase B 18 Chromeleon B 5 B 15 Columns B 23 Computer B 4 EGC vent line B 37 B 53 B 61...

Page 385: ...Local Mode C 10 Locked Remote mode C 4 Loop See Sample loop Low pressure limit A 2 M Main power receptacle 2 11 Maintenance 3 21 Daily 3 21 Ongoing 3 21 Weekly 3 22 Yearly 3 22 Manual sample processin...

Page 386: ...6 PEEK Conductivity cell body 2 43 pH modifer See EPM Electrolytic pH Modifier Physical specifications Decibel level A 1 Dimensions A 1 Weight A 1 Piston seals Replacement procedure 5 18 5 19 Pistons...

Page 387: ...ressure limits C 16 Troubleshooting 4 16 Under pressure alarm 4 10 Pump check valve leaking 4 12 Pump components 2 32 Pump degas See Vacuum degas assembly Pump head waste valve Leaking 4 13 O ring rep...

Page 388: ...AS50 configuration properties B 21 Starting 3 4 B 13 Timebase B 18 Service chase 2 6 2 9 Service procedures 5 17 Check valve cleaning 5 16 5 17 Check valve replacement 5 16 Conductivity cell replaceme...

Page 389: ...panel System overview 1 4 System properties dialog box B 19 T Technical Support 4 1 5 1 Temperature Cell heater 2 43 C 9 Column heater 2 8 2 42 C 8 Minimizing the effect of variations 2 43 Temperature...

Page 390: ...tions Isolating a restriction 4 9 5 10 TUV 1 8 U Unpacking B 2 Computer B 4 USB cable B 11 USB connections 2 10 B 10 B 11 V Vacuum degas assembly 2 30 Calibration 5 9 Components 2 30 Fails to run 4 23...

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