Thermo Scientific Dionex ICS-2000, Operator'S Manual

Page: 373 / 390

Doc. 031857-03 4/06
G-1
G • Glossary
Analytical Column
Synonymous with Separator Column .
Band Spreading
The broadening of the sample band as it travels through the column. Band
spreading can also occur in the injection valve, detector cell, and interconnecting
tubing.
Calibration Curve
A graph showing detector response in peak height or area versus analyte
concentration.
Capacity Factor (k´)
The number of column volumes of eluent, pumped through the column, required
to elute an analyte. Capacity factor is a dimensionless measure of retention which
is independent of column length or eluent flow rate. It is calculated as follows:
Where: t
r
= retention time
t
o
= retention time of unretained solute (column void volume)
k
′
t
r
t
o
–
t
o
-------------- =