Thermo Scientific Dionex ICS-2000, Operator'S Manual

Page: 279 / 390

Setting Up the Eluent Generator
Doc. 031857-03 4/06
B-75
7. Verify that the Automatically turn on with pump option is not
selected on the ICS-2000 touch screen
SUPPRESSOR
page (see
Section C.9 ). Disabling this option lets you turn on the pump, without
automatically turning on the suppressor.
8. Set the pump flow rate to the rate required for the application.
9. Turn on the pump.
10. Verify that the system backpressure is between 14 and 16 MPa (2000
and 2300 psi). If necessary, adjust the pressure by adding or removing
backpressure tubing.
11. Set the
EGC-1
and
EGC-2
concentrations to the values required for
your application. For example, for a 3.5 mM K
2
CO
3
/1.0 mM KHCO
3
mixture, set the
EGC-1
concentration to 3.5 mM and the
EGC-2
concentration to 1.0 mM.
12. Turn on the
EGC-1
and
EGC-2
currents.
13. Run at the selected settings until the mixer is filled with the eluent
mixture. At a flow rate of 1 mL/min it will take about 45 minutes to
fill the 4-mm mixer and about 15 minutes to fill the 2-mm mixer.
NOTE The void volume of the 4-mm mixer is about
16 mL. The void volume of the 2-mm mixer is
about 5 mL.
14. Turn off the
EGC-1
and
EGC-2
currents.
15. Turn off the pump.
16. Remove the yellow PEEK backpressure coil from the
TO INJ VALVE
IN - P/ELUENT OUT
line and the mixer inlet.
17. Disconnect the mixer outlet from the
TO SRS/AES REGEN OUT
line
and remove the 10-32 to 1/4-28 coupler.
18. Reconnect the
TO SRS/AES REGEN OUT
line to the suppressor
REGEN
OUT
port.
To avoid damaging the suppressor, always turn off the suppressor
before filling the mixer. The pump flow is on during filling, however,
no flow reaches the suppressor.