ProteOn XPR36, User Manual

Page: 45 / 288

ProteOn XPR36 Instrument
27
To exit the Fault state, do one of the following:
• Click Reset to attempt to return to the Ready state. This causes the
instrument to attempt initialization operations
• Eject the chip and return to the Power Up state
If a critical error occurs while initializing, the state changes to Fault. If a
noncritical error occurs, a warning appears, but the state changes to Ready.
Because initializing may take a few minutes, the status bar displays Initializing.
The state changes to Ready after initialization finishes.
F
LUSHING
S
TATE
While the instrument fluidics are being flushed, the status bar displays
Flushing.
Buffer System
F
LUSHING AND
P
RIMING THE
S
YSTEM
Flushing the system pumps the selected buffer at the maximum flow rate
through the instrument fluidics, bypassing the flow cell
(buffer does not flow
over the sensor chip). The flushing process replaces the previous buffer in the
fluidics with the new buffer and eliminates air bubbles in the system. Flushing
can be performed using either the buffer control buttons on the instrument or
the Valve/Flush controls in the Instrument Control screen of ProteOn Manager
software.
See Flushing the System on page 67.
Priming the system pumps the selected buffer through the instrument fluidics
and through the flow cell. Priming is a function in the maintenance protocol,
performed with an MNT chip inserted in the instrument. Priming flushes and
pumps buffer over the surface of the chip. Buffer flows through the instrument
fluidics at the maximum rate and through the flow cell at a rate compatible
with the flow cell. The priming function fills the buffer system with new buffer,
eliminates air bubbles in the system, and prevents signal drift resulting from
the slow change in refractive index during the transition between buffers. The
system can be primed only on the Maintenance screen in ProteOn Manager
software.
See Priming the System on page 66.