ProteOn XPR36, User Manual

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ProteOn XPR36 | Introduction
28
S
ELECTING
B
UFFER
B
OTTLES
The two ProteOn XPR36 instrument buffer bottles can hold either identical or
different fluids. At any given time, the fluidics system is connected to a single
source. The fluids for both syringe pumps flow from a single buffer bottle.
Either or both buffer bottles can be removed for refilling or replacing while the
pumps are stopped. The buffer fluids can also be replaced between
experiments or while an experiment is paused. After the buffer source is
changed, the system must be flushed. Between bottle changes, always rinse
the pickup filters by dipping them in distilled water (approx. 50 ml) to prevent
cross-contamination of the liquids.
• The flow of one buffer can be stopped and the other buffer
selected manually by using the analog (manual) buffer control
buttons on the instrument or on the Instrument Control screen in
ProteOn Manager software
Flushing the System with Instrument Control Buttons
The three buffer control buttons are active when the system is in Ready state.
In this state, the system is prepared to start an experiment, maintain
temperature control, and direct buffer to all channels at the default flow rate.
In other states, the buttons are inactive, but the protocol can be stopped to go
to a state in which the buffer fluids can be changed.
Press the button directly below a bottle (Buffer A or Buffer B) to select it as the
buffer source and flush the entire fluidics system except for the flow cell. The
two selection valves control the flow of buffer. Press Stop to stop the flow
from the selected bottle.
After pressing Stop, allow 5 sec for valve switching to take place before
pressing a buffer control button on the instrument or clicking Valve/Flush in
the software.
Caution: Do not allow buffer bottles to run dry.
If you are not using Bio-Rad-manufactured ProteOn buffers, you
must run the buffers through a 0.22 μm filter.