ProteOn XPR36, User Manual

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ProteOn XPR36 System | Glossary
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Injection Process used to introduce a sample into the flow cell starting with a
needle wash, followed by sample withdrawal, sample injection, and
another needle wash
Injection Alignment Process of aligning the start of multiple injections generated either by
quasi-simultaneous sample injections or sequential sample injections
Injection Quality Parameter set to control the number of bubbles separating the
injected sample from the running buffer in an injection step. Options
are: minimum (1 bubble), medium (2 bubbles), and maximum
(3 bubbles)
Injection Time See Contact Time
Instrument Settings Global instrument settings that include the autosampler temperature,
sensor chip temperature, default flow rate, and standby flow rate.
The default flow rate and the standby flow rate are not user editable
Interaction See Biomolecular Interactio n
Interaction Analysis Analysis of biomolecular interaction data (kinetics, equilibrium, etc.)
using the ProteOn XPR36 system
Interaction Array Arrangement, in 6 x 6 format, of 36 interaction spots formed when six
analyte samples flow orthogonally over six channels of immobilized
ligand on the surface of the sensor chip, in the ProteOn XPR36
instrument
Interaction Spot One of up to 36 sites on the ProteOn XPR36 sensor chip surface
where a biomolecular interaction is measured
Intercept Intersection of the data with either axis (X or Y) of the graph
Interspot Reference One of 42 spots within the analyte channels used for referencing.
These are sites that were not exposed to ligand during the
immobilization process. Interspot references can be used in place of
or as a complement to a reference channel
Instrument ProteOn XPR36 system component that houses the microfluidics
system, optical detection system, tubing, and associated controls
etc., for the purpose of interaction analysis
k
a
See Association Rate Constant
k
d
See Dissociation Rate Constant
K
D
See Equilibrium Constant
Kinetics Reaction rate, or rate of change, of a reaction or interaction
Langmuir Simple 1:1 bimolecular interaction
Langmuir Mass
Transfer Simple 1:1 bimolecular interaction, in which analyte diffusion to the
surface is slower than the interaction itself