ProteOn XPR36, User Manual

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ProteOn XPR36
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3
Experiments
After opening or creating a protocol, you can run an interaction experiment.
After running an experiment, you can process raw data for analysis. See
Chapter 4, Analysis.
Required Materials
The following equipment, samples, and reagents are necessary to perform
interaction analysis using the ProteOn™ XPR36 system:
• ProteOn XPR36 instrument and computer running ProteOn
Manager™ software
• Ligand and analyte
• ProteOn sensor chip
• ProteOn amine coupling kit (required for GLC, GLM, and GLH
sensor chips)
• Immobilization buffer (for example, ProteOn acetate buffer pH 4.0,
4.5, 5.0, or 5.5)
• Running buffer (for example, ProteOn phosphate buffered saline
with 0.005% Tween 20, pH 7.4)
• Conical centrifuge tubes, 50 ml
• ProteOn sample vials with custom sample rack or ProteOn
microplates
• Pipets with tips
• ProteOn microplate sealing film
Caution: Using sample vials other than the
ProteOn sample vials may damage the needles
used to load the samples.