ProteOn XPR36, User Manual

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ProteOn XPR36 System | C Surface Plasmon Resonance
224
Regeneration Step
If an immobilized ligand surface is going to be used in another set of
experiments, any remaining bound analyte must be removed first. In some
cases, particularly with small-molecule interactions, this can be accomplished
after the dissociation phase, simply by flowing buffer long enough for
complete dissociation to occur. If this is insufficient, or removal takes too long
to be practical, the remaining analyte is removed using a regeneration step in
which acidic, basic, ionic, or detergent-containing buffer is passed over the
sensor chip surface.
For effective regeneration, the buffer must be able to strip off the bound
analyte, but maintain the activity of the immobilized ligand. Use high flow
rates and minimal volumes (100 μl/min and 30 μl, for example) to minimize
damage to the ligand. The ProteOn Regeneration Buffer Kit contains nine of
the most commonly used regeneration solutions.