ProteOn XPR36, User Manual

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ProteOn XPR36 System | Glossary
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Double Reference Correction of analyte data using a blank injection as the reference.
Prior to applying the double reference, both the blank and analyte
data are corrected using interspot or channel referencing. Sensor
chip background is interspot reference and background contributed
by biological (experimental) sources (for example, buffer without
analyte). Subtracting both backgrounds is double referencing
Dynamic Range Upper and lower bounds on the ability of a system (that is,
instrument, reagents, etc.) to measure. In the context of interaction
analysis, it refers to the range of SPR signals which the ProteOn
XPR36 system can measure. Dynamic range is expressed in
response units
EDAC Also abbreviated EDC, 1-ethyl-3-(3-dimethylaminopropyl)
carbodiimide hydrochloride is used for sensor chip surface activation
(see
Activation )
Equilibration See Stabilization
Equilibrium Constant (K
D
), also known as the equilibrium dissociation constant,
characteristic of a reversible chemical reaction, measured in units of
molarity, or M. It relates the concentrations of all reactants and
products at equilibrium. It is calculated from the ratio of the
dissociation and association kinetic rate constants, such that
K
D
= k
d
/k
a
Evanescent Wave Electromagnetic wave generated by a light wave in total internal
reflection at the interface of two media. It decays exponentially with
distance as it passes into the medium of lower refractive index
Excluded Volume
(EV) Correction Use of high refractive index cosolvents, such as DMSO, in SPR
results in a differential bulk response between the chip's reference
surface and the interaction surface. This is due to the cosolvent
being excluded from the chip surface by the ligand and results in a
larger bulk response on the reference surface than on the interaction
surface. If not corrected, this excess response results in an error
when subtracting the reference data from the interaction data. The
excluded volume correction function calculates the excess response
from a calibration curve and then removes the excess response from
the reference data