ProteOn XPR36, User Manual

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Analyzing Data
127
• Concentration analysis – determines the concentration of analyte
in samples based on a set of standard samples
If you want to exit a wizard, click Save to save the analysis data. Saving the
data saves a partial analysis, which appears as a gray underlined title under
the corresponding dataset.
K
INETIC
A
NALYSIS
W
IZARD
To run the Kinetic Analysis wizard, select a dataset on the Analysis Sets
screen and follow the steps of the wizard.
Step 1: Biochemical Model Selection
Seven models are available for fitting kinetic data. The most commonly used
is the Langmuir 1:1 model.
Other models are used under special circumstances.
In the pull-down list, select a kinetics calculations model from the following
options, and then click Next:
• Langmuir — simple 1:1 biomolecular interaction. Langmuir 1:1
ratio is the most common kinetic fit model used for SPR data
analysis. The reaction is between the ligand (L) immobilized to the
surface of the chip and the analyte (A) that is flowed across it. As
the analyte interacts with the ligand, a complex is formed following
pseudo first-order kinetics. When the analyte is washed away from
the chip, the two molecules dissociate. It is assumed that the
binding is equivalent and independent for all binding sites. It is also
assumed that the reaction is not limited by mass transport. Most
SPR reactions adhere to this model.