ProteOn XPR36, User Manual

Page: 114 / 288

ProteOn XPR36 System | Analysis
96
V
IEWING
K
INETIC
D
ATA IN AN
I
SOAFFINITY
G
RAPH
The isoaffinity graph enables you to visualize kinetics by plotting together the
association and dissociation values in such a way that conclusions can be
drawn from viewing all the different molecules. The x-axis displays association
(k
a
), and the y-axis displays dissociation (k
d
). The isoaffinity (K
D
) appears on
the diagonal line. You can change the labels of the axes using the scale boxes
near the bottom of the graph. You can also change the title using the Title box
at the bottom of the graph.
You can select input data for the isoaffinity graph from multiple datasets and
analyses within the current experiment by selecting groups or individual points
in the left pane of the Isoaffinity Graph dialog box.
To view an isoaffinity graph:
1. Open an experiment that includes a kinetic analysis.
2. In the navigation panel, click Analysis Dataset and select a kinetic
analysis.
3. Choose Isoaffinity Graph on the Analysis menu. The graph appears in a
separate window.
Default k
d
and k
a
scale values appear in the drop-down lists at the bottom of
the isoaffinity graph dialog box.