ProteOn XPR36, User Manual

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Excluded Volume
(EV) Effect Residual bulk effect that is not referenced out by the use of a
reference channel with different surface chemistry and/or ligand
density. It is caused by more bulk solvent being present near the chip
surface in the reference channel than in the sample channel. This
results from the bulk solvent being partially excluded from the chip
surface by the ligand in the sample channel. This effect can generally
be ignored, unless a high refractive index buffer (for example, a buffer
with DMSO in it) is being used with a reference channel with a very
different ligand density
Experiment Series of operations carried out to measure and record kinetic,
equilibrium, or concentration data. Recorded experimental data is
referred to as an "experiment" and consists of a protocol and chip
data. It also usually contains raw data, processed data, and analysis
data. An experiment file is saved with a *.pxf extension
Fit To adjust (a smooth curve of a specified type) to a given set of points
Flow Cell For the ProteOn XPR36 system, the fluidic channels formed when the
multichannel module is pressed onto the chip surface
Flow Rate Rate of fluid flowing through channels in units of volume per unit time
Flush Pumping the selected buffer at the maximum flow rate through the
instrument fluidics, bypassing the flow cell (buffer does not flow over
the sensor chip). The flushing process replaces the previous buffer in
the fluidics with the new buffer and eliminates air bubbles in the
system. Flushing takes 10 min
Global Scope assigned to a parameter, meaning only one value for all the
data will be fitted
Grouped Scope assigned to a parameter, meaning only one value for each
group of the sensorgrams will be fitted
Heterogeneous
Analyte Interaction model in which two analytes compete for binding to a
single ligand site
Heterogeneous
Ligand Interaction model in which one analyte binds two different ligand
species
Immobilization Process of covalently binding a ligand or capture agent to an
activated sensor chip surface
Immobilization
Group Set of steps performed to activate a sensor chip, bind a ligand to the
chip, and deactivate the chip
Immobilization
Level Amount of ligand immobilized on the sensor chip surface. It is
expressed in response units, RU
Initialization Process run by the instrument to prepare the detection and fluidics
systems for an experiment