ProteOn XPR36, User Manual

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ProteOn XPR36 System | Analysis
112
opens that guides you through performing the calibration. See
Processing EV Correction Data on page 115
Double Referencing — Perform double referencing by collecting interaction
data using a blank sample (double reference data). These data include
artifacts that result from the ligand surface, such as drift due to ligand or
nonspecifically bound protein leaching from the chip surface.
The typical double referencing approach when using the ProteOn system is to
collect double reference data in real time as part of the analyte injection itself
(Row Reference). If needed, double reference data can be collected as part of
a separate injection step (Injection Reference).
Select Double Reference on the Process menu and choose the type of double
reference to use.
• Row Reference — up to 5 analytes are injected along with 1 blank
sample (row reference sample). The blank reference can be in any
channel. The row containing the double reference can be selected
manually, or if the double reference sample was defined with the
Blank sample type, selecting the Blank option automatically uses it
as your row double reference. It’s a good idea for the analyte
sample panel to contain only one sample with the blank sample
type. The row reference data are subtracted from the analyte data
collected in the other 5 rows
• Column Reference — In situations where analytes are run in the
vertical direction, a column double reference can be used. Up to 5
analytes are injected along with 1 blank sample (column reference
sample). The blank reference can be in any channel. The column
containing the double reference can be selected manually or if the
double reference sample was defined with the Blank sample type,
selecting the Blank option automatically uses it as your column
double reference. It’s a good idea for the analyte sample panel to
contain only one sample with the Blank sample type. The column
reference data are subtracted from the analyte data collected in the
other 5 rows
• Blank Reference — when one of the analyte samples is defined as
Blank type (in the Protocol Samples tab), ProteOn Manager
software finds it and uses it as your double reference. This is useful
when processing together several analyte steps; for each of them
the Blank sample is located in a different channel. If more than one
sample in a step is defined as Blank, the first one is used for
referencing