ProteOn XPR36, User Manual

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Analyzing Data
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• Global — calculates one value for all sensorgrams
• Local — calculates a different value for each sensorgram
• Grouped —calculates one value for each sensorgram group (all the
sensorgrams within a data window)
Fit/Constant define how values are assigned:
• Fit — is determined during the numerical fit process as the best
value to fit the biochemical model selected
• Constant — is held constant during the numerical fit process
S
PECIFYING
R
EPORT
P
OINTS
Specifying report points on sensorgrams enables you to compare response
unit (RU) values before the end of an injection (for signal level at equilibrium),
or after the immobilization of the ligand (for ligand immobilization
calculations). Report points are usually calculated after pre-processing—after
you have
performed reference subtraction and baseline alignment to zero. You
can generate report points from raw or processed data.
Creating a report point adds columns to the results table. Report point
columns are added in order by time and are defined in terms of their position
relative to the start of injection. Each report point adds three columns to the
table: value being returned; start time; and end time.
Note: You can create report points in the Data screen. However, if
data in the data table are replaced, any report points associated
with the data in the data table are also replaced. The data are
saved only when you create a dataset.
When you create a report point in an analysis dataset, it is saved permanently
as part of the dataset.
To specify a report point from a dataset:
1. Select a range on a sensorgram by right-clicking a selected sensorgram
and dragging the range.
2. Click Create Report Point on the toolbar to open the Create New Report
Point dialog box.