ProteOn XPR36, User Manual

Page: 149 / 288

Analyzing Data
131
It is possible to change the starting parameters for the fit calculation by typing
the desired values in the table. When you want to use a pre-determined value
for one of the fit parameters, change the Fit Type of this parameter to
Constant, and type the requested value in the table. For example, pre-
determined R
max
could be set to the theoretical R
max
value in low affinity
models, when there is no or limited curvature in the fitted sensorgrams. See
Immobilization Step on page 216 for theoretical R
max
calculation.
Step 3: Results Preview
When you click Next, the wizard calculates the fit. Once the fit is calculated,
the following table appears with the Report tab open.
Analyzed datasets show sensorgrams and their fitted curves in data windows.
Icons appear in the left margin of the dialog box.
• Report — opens the table summarizing numerical results of the
analysis
• Residual — opens the kinetic analysis residuals graph, which plots
the calculation residuals along the y axis and experiment time
along the x axis. Residuals are a graphical representation of the
fitting accuracy and appear only for the sensorgrams selected with
the Interaction Display Chooser during data preprocessing