ProteOn XPR36, User Manual

Page: 89 / 288

Sensor Chips
71
Sensor Chip Types
Type
(and Color)
Chemistry Applications
GLC
(magenta) Compact polymer layer containing easily
activated carboxylic groups for general amine
coupling. The compact planar structure yields a
ligand capacity of about one monolayer Kinetic analysis
Equilibrium analysis
Concentration determination
Immobilization scouting
Regeneration scouting
Analyte screening
GLM (blue) Polymer layer containing easily activated
carboxylic groups for general amine coupling
that yields moderately increased ligand
capacity with respect to GLC Same as for the GLC chip, but is especially
well-suited for work with applications that
require a higher ligand immobilization level
GLH (orange) Polymer layer containing easily activated
carboxylic groups for general amine coupling
that yields very high ligand capacity Same as for the GLM chip but is especially
well-suited for work with small molecules
or other applications that require a very
high ligand immobilization level
NLC (yellow) NeutrAvidin layer attached to a GLC chip for
binding of biotinylated ligand molecules Immobilization of biotinylated molecules
for:
Kinetic analysis
Equilibrium analysis
Concentration determination
HTG (red) Polymer layer containing tris-NTA complexes
for capturing His-tagged proteins Immobilization of His-tagged proteins for:
Kinetic analysis
Equilibrium analysis
Concentration determination
Selective capturing of the His-tagged
proteins directly from crude media is
possible
HTE (red) Polymer layer containing tris-NTA complexes at
elevated density for higher binding capacity; for
capturing His-tagged proteins Same as for the HTG chip but especially
suited for work with small molecules or
other applications that require high ligand
immobilization level
MNT (black) None Running maintenance protocols. See
Maintenance Chips (MNT) on page 150 for
more information
CLN (green) Adhesive Cleaning the MCM. See Cleaning Chips
(CLN) on page 151 for more information