ProteOn XPR36, User Manual

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ProteOn XPR36 | Experiments
76
Default Injection Quality and Needles Washes
Samples Layout
Initializing the Chip
Any type of chip or experiment can use any of the three initialization options
shown on the Instrument Control screen. Whether to use air or glycerol is a
matter of personal preference. Rules govern when each method can be used.
• Air initialization —This button is enabled only the first time a
particular sensor chip is inserted
• Glycerol initialization —You can use glycerol initialization for new or
used chips
• Use Last initialization — May be used only when reusing a sensor
chip, such as after a software-only shutdown to change users. This
button is disabled once a chip is replaced with a different chip until
a new air or glycerol initialization is performed
Inserting and Ejecting the Sensor Chip
To insert the chip:
1. Cut open the pouch containing the temperature-equilibrated ProteOn
sensor chip and insert it into the instrument chip loader. The chip ID,
Step Type Orientation Composition Volume,
μ
l
1 Regeneration H 0.5% SDS 30
2 Regeneration H 50 mM NaOH 30
3 Regeneration H 100 mM HCI 30
4 Regeneration H 300 mM EDTA, pH 8.5 100
5 Regeneration V 0.5% SDS 30
6 Regeneration V 50 mM NaOH 30
7 Regeneration V 100 mM HCI 30
8 Regeneration V 300 mM EDTA, pH 8.5 100
Solution Volume Rack Configuration Microplate Configuration
0.5% SDS
200
μ
l
L1–L6 H1–H6
50 mM NaOH
200
μ
l
K1–K6 G1–G6
100 mM HCl
200
μ
l
J1–J6 F1–F6
300 mM EDTA, pH 8.5
350
μ
l
I1–I6 E1–E6