ProteOn XPR36, User Manual

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ProteOn XPR36 | D Protocol Development Kits
226
Protein–Small Molecule Kit
The ProteOn protein–small molecule kit provides the materials for kinetic
analysis of the interaction between an enzyme (carbonic anhydrase II) and its
substrate (4-carboxybenzenesulfonamide, or CBS). CBS is a carbonic
anhydrase inhibitor with a molecular weight of 201 Da. The carbonic
anhydrase II/CBS interaction not only demonstrates the ability of the ProteOn
XPR36 system to detect low molecular weight analytes, but it also highlights
the methodology for optimizing system performance.
The system measures the interaction kinetics of the enzyme/inhibitor pair
through the immobilization of ligand (carbonic anhydrase II) on the surface of
a sensor chip, followed by injection of an analyte (CBS) concentration series in
six orthogonal channels. Each interaction produces a sensorgram for detailed
kinetic analysis and validation testing.