ProteOn XPR36, User Manual

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ProteOn XPR36 System | Analysis
132
To save the analysis data:
• Click Finish. The analysis is saved in the experiment and appears
listed under its corresponding dataset.
To save the data to a file:
• Copy and paste or export the data.
E
QUILIBRIUM
A
NALYSIS
W
IZARD
Equilibrium analysis derives the equilibrium parameter K
D
from a set of
sensorgrams that have reached a steady state.
Select a dataset on the Analysis Datasets screen and follow the steps in the
wizard.
During any step of the wizard, you can click Save to close the wizard and save
the data you have accumulated. Saving the data saves a partial analysis,
which appears listed under the corresponding dataset.
Step 1: Region Selection for R
eq
Calculation and Analysis Parameters
Select sensorgram regions to be used to calculate K
D
. If necessary, use the
Zoom In tool to examine each sensorgram in more detail. To select a
sensorgram region, drag the vertical bars in the sensorgram. Alternatively, the
Begin and End times of the equilibrium (the fit region) can be changed by
typing the values in the table.
When performing Equilibrium analysis, it is important to use data in which the
responses of all analyte concentrations have reached equilibrium and to
confine the fit region to the areas where the signals are flat. The vertical bars
define the start and end regions in the sensorgram used to calculate the
equilibrium dissociation constant (K
D
). R
eq
response at equilibrium is
determined by the maximum number of binding sites (R
max
) and is calculated
from the data segment between the vertical bars on the sensorgram.