ProteOn XPR36, User Manual

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ProteOn XPR36 System | Protocol Design
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Protocol Step Details — lists protocol types, some of which can be changed.
The appearance changes depending on the type highlighted. If a step group is
highlighted, the name and type appear. If a step is highlighted, the details in
the following table appear (depending on the step type).
Detail Description
Step Types Allows you to select Activate, Ligand, Deactivate, Regenerate, Analyte,
CoInject Ligand, CoInject Analyte, Pause, Set Temperature, Set Buffer,
and Change Rack
Step Name Applies when a new step is added to the Protocol Steps list. The step is
given the name “Step Type +
n
,” where
n
indicates that the step is the
n
th step of that type created. You can change the name in the Step
Name box
Flow Rate Rate at which fluid flows through the channels. This parameter applies
to all stages of a step. If this value is changed, contact time is
recalculated using the current volume
Contact Time Amount of time that an injected sample is in contact with the sensor
chip surface. Contact time = injection volume/flow rate. If this value is
changed, volume is recalculated at the given flow rate
Volume Equals the volume of the injected sample. If this value is changed,
contact time is recalculated at the given flow rate
Dissociation The time period when a biomolecular complex is allowed to dissociate
into its separate components. This parameter appears in the Analyte,
CoInject Analyte and Blank injection steps, as well as in the Advanced
settings dialog box
Advanced Overrides default settings for each step. To edit the parameters, clear
the Use Default checkbox