ProteOn XPR36, User Manual

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ProteOn XPR36 System | Glossary
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Normalization Standardization of the detector response at total internal reflection
over the entire 6 x 6 interaction array
One-shot Kinetics™
(OSK)™ Detailed interaction analysis experiment using six ligand
immobilization levels and six concentrations of analyte on a single
sensor chip and in a single injection cycle
Optical Detection
System The light source, the collection lenses, the polarization optical
element, the biosensor chip (prism), and the detector in the ProteOn
XPR36 instrument
Orthogonal Intersecting perpendicularly
Panel Set of up to six samples or reagents applied to the sensor chip in a
single injection. During immobilization, for example, a panel of up to
six ligands may be immobilized on the chip (one in each channel)
Preconcentration Process of attracting ligand to the sensor chip surface prior to
covalent immobilization on the surface of the chip. This is usually
accomplished through electrostatic interactions
Preconditioning See Baseline Stabilization
Prime To pump a solution through the microfluidics system and across a
chip. Prime performs a fluidics system flush (see
Flush ) at the
maximum flow rate, and then pumps buffer through the flow cell at a
flow rate compatible with the flow cell. Prime is used to replace the
buffer in the fluidics system with a new buffer, and to remove air
bubbles from the fluidics system. Priming takes 20 min
Protocol Parameters and steps that define how an experiment is performed.
Protocols can be saved as a separate file and may be associated
with an experiment or a standalone file (*.prt)
Rate Constant Proportionality constant in the relationship between reaction rate and
concentrations of reactants
Ready State ProteOn XPR36 instrument mode in which an experiment can be
started. In this state, temperature is maintained, and the default
buffer flows through all the channels at the default flow rate
Reference
Channel
One of the six ligand flow cell channels that does not bind the
analyte, due to the chip surface either not having an immobilized
ligand on it or having an inactive ligand on it. During data processing,
data collected from this channel is subtracted from data collected in
the other five channels to remove injection artifacts (see
Reference
Subtraction )