ProteOn XPR36, User Manual

Page: 93 / 288

Sensor Chips
75
NLC S
ENSOR
C
HIP
C
ONDITIONING
Note: Trehalose is used as a protective layer for the dry
NeutrAvidin on the chip surface. We believe the trehalose is
completely removed by continuous buffer flow over the chip
surface. However, adding one or two blank buffer injections using
chip conditioning prior to the first expermental injection should
ensure complete removal.
Buffer: Use the same buffer you prefer for running experiments, but when
working with buffers containing metal ions, eliminate the NaOH injections.
Flow Rate: 30
μl
/min
Default Injection Quality and Needles Washes
Samples Layout
HTG S
ENSOR
C
HIP
C
ONDITIONING
Buffer: Use your preferred running buffer, but when working with buffers
containing metal ions that form hydroxide precipitates, eliminate the NaOH
injections.
Flow Rate: 30
μl
/min
Step Type Orientation Composition Volume,
μ
l
1 Regeneration H 1 M NaCl 30
2 Regeneration H 50 mM NaOH 30
3 Regeneration H 1 M NaCl 30
4 Regeneration H 50 mM NaOH 30
5 Regeneration V 1 M NaCl 30
6 Regeneration V 50 mM NaOH 30
7 Regeneration V 1 M NaCl 30
8 Regeneration V 50 mM NaOH 30
Solution Volume Rack Configuration Microplate Configuration
1 M NaCl
350
μ
l
L1–L6 H1–H6
50 mM NaOH
350
μ
l
K1–K6 G1–G6