ProteOn XPR36, User Manual

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Sensor Chips
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If the temperature is out of range, the actual temperature appears in red.
When an experiment starts, the actual temperatures are validated against the
protocol temperature and a warning appears if there is a mismatch. This does
not prevent the experiment from continuing.
Reusing Chips
If a chip has had ligand immobilized on fewer than six channels during its
previous insertion, you can reinsert the chip and immobilize ligand on the
unused channels. Unused channels are those that have not been exposed to
reagents or conditions that would interfere with subsequent ligand
immobilization. Chips that are being considered for reuse must be kept free of
dust and other contaminants. Any contaminants that collect on the chip’s
optical surfaces will add noise to the data and may result in the MCM needing
to be cleaned.
To run an experiment on a previously used chip:
1. Insert the chip into the instrument.
2. Initialize the chip and select an experiment or protocol to run.
You can then continue the last experiment that was run on the chip, or you
can start a new experiment.
Chip reuse is generally not recommended, except as described above, for the
following reasons:
• Chip channels that have been exposed to reagents (such as
activation reagents) or conditions that would interfere with ligand
immobilization often give unsatisfactory results
Warning! NLC chips cannot be reused after
being ejected from the instrument, even if the
channels are unused. New NLC chips are coated
with trehalose to stabilize the NeutrAvidin
surface and the trehalose coating is completely
removed by buffer flow over the chip surface in
the first insertion.