ProteOn XPR36, User Manual

Page: 103 / 288

Running Experiments
85
Figure 2. CA II/CBS interaction data with excluded volume correction.
To run an experiment with highly refractive cosolvent:
1. Flush the instrument with running buffer without the cosolvent in it.
Cosolvent should not be used in the running buffer unless it is known
that it will not affect ligand immobilization.
Do not allow the buffer bottle
to run dry.
2. Immobilize the ligand onto the sensor as required by the chip type.
3. Make a stock solution of the analyte at the highest concentration to be
used in the experiment. The cosolvent should be at a concentration that
is known to keep the analyte soluble.
4. Prepare running buffer with the same concentration of cosolvent as the
stock analyte solution.
5. Use the running buffer containing the cosolvent to make the remaining
analyte dilutions.
6. Flush the system with running buffer containing the cosolvent.
7. Add any required regeneration, blank, and analyte steps to the protocol.
Note: Blank injections that are used for double referencing must
be made from the running buffer with the cosolvent.
8. In the Protocol tab, drag the EV correction step group to the end of the
protocol. The EV correction step group contains six injections by
default. Up to three of these steps may be deleted but the EV correction