ProteOn XPR36, User Manual

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ProteOn XPR36 System | Protocol Design
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or well volume. If the sample panel volume exceeds the well volume,
the Required Volume box on the Samples screen turns red as a
warning.
Note: After you select Start on the Run screen, the autosampler
parameters cannot be changed.
When Rack is selected, only the Vial box appears.
When Microplates is selected, only the Well box appears.
6. Use the Step Default Injection Parameters area on the Protocol
Configuration screen to display or edit the default injection parameters
for each injection type.
Default parameters are applied globally to each protocol step for a
given injection step type. You can also set parameters for individual
protocol steps by clicking the Advanced link for a step in the Step
Details panel on the Protocol Steps screen.
You can change parameters before the step is executed. You cannot
change injection parameter settings after the experiment starts.
Flow Rate box — sets the default flow rate (25–200 μl/min) associated
with each step type
Volume box — sets the default injection volume (0–465 μl) associated
with each step type
Flow Stabilization box — sets a delay period just before sample
injection. The delay allows the microfluidics system to stabilize after the
flow rate is changed
Dissociation box — sets the default dissociation time for each step
type
Injection Quality menu — sets the number of bubble separators
(minimum = 1, medium = 2, or maximum = 3 bubbles)