ProteOn XPR36, User Manual

Page: 91 / 288

Sensor Chips
73
4. Hold the black end of the cartridge up inside the pouch to ensure that
the sensor chip slide remains inside the cartridge.
5. Press the sensor chip slide firmly into place within the cartridge.
6. Remove the sensor chip cartridge from the aluminum pouch.
Conditioning the Sensor Chip
A set of conditioning protocols and an example protocol are available at the
following location on your computer on the hard drive you selected: \Program
Files\Bio-Rad\ProteOn Manager\Chip Conditioning and Example Protocols.
You can load and save the protocols as templates by selecting File > Save As
Template in ProteOn Manager software.
The sample protocols can be used to condition sensor chips with Bio-Rad’s
recommended settings. These files can also be modified to meet your needs.
The following procedure is recommended for new sensor chips and can
provide:
• General improvement of data quality
• Rapid stabilization of the baseline prior to starting the experiment
GLC, GLM,
AND
GLH S
ENSOR
C
HIP
C
ONDITIONING
Buffer: Use the same buffer you prefer for running experiments, but when
working with buffers containing metal ions that form hydroxide precipitates,
eliminate the NaOH injections.
Flow Rate: 30
μ
l/min
Cartridge
Sensor chip slide