ProteOn XPR36, User Manual

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Reference
Subtraction Baseline operation performed on sensorgrams to remove
contributions made to the SPR by phenomena not linked to the
interaction under evaluation (for example, temperature, nonspecific
binding, and bulk effects). With the ProteOn XPR36 system,
reference subtraction can be performed using interspot references
(see
Interspot References ) or a reference channel (see Reference
Channel )
Refractive Index Factor by which the phase velocity of electromagnetic radiation is
slowed in that material, relative to its velocity in a vacuum. It is also
known as the index of refraction
Refractive Index
Unit (RIU)
Equal to refractive index increment of 10
e
-6
Regeneration Preparation of the sensor chip surface for measuring interaction data
using a new set of interaction conditions after an interaction has
been carried out
R
eq
Response at equilibrium, determined by the maximal amount of
binding places (R
max
), kinetic constants (k
a
, k
d
), and analyte
concentration
Residual Difference between the processed interaction data (sensorgrams)
and the calculated regression curve of the data
Response Any change in the SPR signal
Response Unit (RU) Quantitative expression of the SPR signal describing mass
concentration at the chip surface. It is equal to 10
e
-6
of 1 RIU
R
low
Estimated response at zero concentration in RU units
R
high
Estimated response at infinite concentration in KU units
R
max
Maximal number of binding places in RU units
Running Buffer Continuous-flow solution used to transport interactants to and from
the sensor chip surface and to keep solution flowing over the chip
between sample injections
Running State ProteOn XPR36 instrument mode in which an experiment is being
executed
Sample Holder Generic term referring to the microplates or sample rack used to hold
samples in the autosampler (see
Microplate and Sample Rack )
Sample Holder
Sensor Component of the autosampler that detects and recognizes the type
of sample holder (microplates or sample rack) being used. There are
three sensors